Email updates

Keep up to date with the latest news and content from BMC Hematology and BioMed Central.

Open Access Highly Accessed Research article

Effectiveness of communication campaign on iron deficiency anemia in Kyzyl-Orda region, Kazakhstan: a pilot study

Ainur Baizhumanova1*, Akio Nishimura2, Katsuki Ito1, Junichi Sakamoto1, Nailya Karsybekova3, Igor Tsoi4 and Nobuyuki Hamajima5

Author Affiliations

1 Department of Young Leaders' Program for Medical Administration and Politics, Graduate School of Medicine, Nagoya University, 65 Tsurumai-Cho, Nagoya, 466-8550, Japan

2 Department of Education Training, Technology and Development, National Institute of Public Health, 2-3-6 Minami, Wako, 351-0197, Japan

3 Department of Alimentary Dependent Diseases, Institute of Nutrition Issues, 49 Beibitshilik Str, Astana, 010000, Kazakhstan

4 Kazakh Academy of Nutrition, National Academy of Sciences, 66 Klochkov Str, Almaty, 050000, Kazakhstan

5 Department of Preventive Medicine/Biostatistics and Medical Decision Making, Graduate School of Medicine, Nagoya University, 65 Tsurumai-Cho, Nagoya, 466-8550, Japan

For all author emails, please log on.

BMC Blood Disorders 2010, 10:2  doi:10.1186/1471-2326-10-2

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2326/10/2


Received:24 February 2009
Accepted:17 March 2010
Published:17 March 2010

© 2010 Baizhumanova et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

In 2004, wheat flour fortification (WFF) with iron was implemented in Kazakhstan as a public health strategy to increase the iron intake of all women of childbearing age and of children. In 2003, before starting the flour fortification program, a communication campaign on health education took place in a region with a high prevalence of iron deficiency anemia (IDA). The present study aimed to evaluate the prevalence of anemia, iron deficiency and IDA before and after the campaign. In addition, knowledge about IDA and its prevention, as well as awareness about fortified wheat flour, was assessed.

Methods

The subjects of the study were women aged 15-49 years and children aged 2-14 years. The study was carried out in urban and rural areas of Kyzyl-Orda region in 2003 before (March) and after (December) the campaign. Blood samples were collected in order to measure hemoglobin and serum ferritin. In March 80 women and 57 children in the urban area, and 41 women and 41 children in the rural area, participated in the IDA testing. The corresponding participants in December numbered 62, 52, 52, and 57, respectively. The impacts of the communications and information received by participants during the campaign was surveyed with a questionnaire for 195 women in March and 198 women in December including some who participated in the IDA testing.

Results

In March, the prevalence of anemia was 52.0% among 121 women and 58.1% among 98 children, and those with low iron reserve were 63.6%, 49.1% and IDA 40.5%, 11.0%, respectively. In December, the prevalence of anemia had significantly decreased among rural women (from 65.9% to 48.0%, p < 0.05) and among urban children (from 63.1% to 11.5%, p < 0.001). The prevalence of iron deficiency was significantly reduced among the children (from 51.1% to 24.8%, p < 0.001). IDA prevalence was meaningfully decreased among women in urban and combined areas (from 37.5% to 15.0% and 40.5 to 14.8%, respectively, p < 0.001) and among urban children (from 7.1% to 2.1%, p < 0.05). The surveys found that most women knew about IDA and its prevention and that the numbers were similar both in March and in December. The knowledge of the anti-anemic effect of wheat fortified flour improved significantly over the period of the campaign among women both in urban (from 48.5% to 80.9%, p < 0.001) and rural (from 69.8% to 88.6%, p < 0.001) areas.

Conclusion

The study demonstrated that the communication campaign before implementation of WFF program was effectively carried out, giving a biological impact on hematological indices.

Background

Iron deficiency anemia (IDA) is the most common and widespread nutritional disorder in Kazakhstan [1-11]. In 2004, as one of the comprehensive public health measures to achieve improvement in the country, a wheat flour fortification (WFF) program took place. Before implementation of the WFF, a communication campaign to build public awareness about IDA and its prevention, as well as about new product fortified wheat flour, was conducted. This campaign consisted of health education through different types of media, social mobilization, and marketing by civil society. The study aimed to evaluate the prevalence of anemia, iron deficiency and IDA before and after the campaign. In addition, improvement of awareness about IDA, its prevention, and fortified wheat flour were assessed.

Methods

Study location and population

Kyzyl-Orda region is situated in southern Kazakhstan, extending along both banks of the lower Syrdarya River. The Aral Karakum and Kyzylkum deserts, making it one of the hottest and most arid regions of Kazakhstan, cover a large part of this region. The total population of the region was about 603.800 in 2003, or about 4 percent of the country's population, and two-thirds of the population was urban. In that year, the region ranked lowest in per capita income, with industrial production representing about 1.1 percent of the country's total production. The principal industry is agriculture, specifically the breeding of cattle, growing rice and fishery. In addition to some social and economic difficulties, the region suffers from environmental pollution, a difficult climate, and high levels of pesticide concentration in the environment and salts of heavy metals. All of these factors negatively affect the health condition of the population. One of the most serious public health problems in the Kyzyl-Orda region is anemia. According to numerous studies the prevalence rate of anemia is the highest in the country [1-11]. Although many causes of anemia have been identified, such as hemoglobinopathies, parasitic infestation, chronic bleeding, and deficiencies of folic acid and vitamin C, evidence suggests that the majority of cases of anemia in Kazakhstan are due to negative iron balance [5].

Study subjects

Kyzyl-Orda was chosen as a pilot region for the present study because the IDA prevalence in this region is the highest in the country. One city and one district were selected from six districts of the pilot region, which allowed for comparison of the differences in efficiency between urban and rural areas. The study consisted of two surveys with a time interval of 9 months. The first survey was conducted in March before the campaign and the second survey in December of the same year after the campaign.

A two-stage sampling process was used to select women for the study. In the first stage, Kyzyl-Orda city and Kazalinsk district were chosen because of the highest prevalence of IDA in the Kyzyl-Orda region. In the second stage, every sixth household was selected from the complete list of households registered at the selected health centres. Participants were selected by systematic random sampling. An initial household was selected at random and every sixth household was chosen from the list of households. The sampling frame was assumed to be complete since all women and children in Kyzyl-Orda region were required to be registered at their local health centre. Women aged 15-49 years and their children aged 2-14 years were invited to the clinics on designated days. The same selection method was used for both urban and rural areas as well as for March and December surveys.

The Ethical Committee of the Kazakh Academy of Nutrition and Ministry of Health of Kazakhstan approved the study. Prior to participating in the IDA testing, each woman was asked to sign a consent form giving permission for the collection of a blood droplet from her and her children.

From 200 households 200 women and 100 children were selected for our study. Of these, 121 women and their children were selected for IDA testing and interview. The remaining 79 women were selected only for the interview. In March, of those eligible women and children selected, approximately 60.5% of women and 98% of children participated in the IDA testing. Ninety seven percent of women attended the interview and that number included the women who participated in the IDA testing. In December the corresponding participation rates were 57%, 100% and 99% respectively. The main reasons of absence of women who did not participate in the IDA testing were: not at home, and/or not available to come to the clinic. Percentages of refusals among women in March and December were 1.0 and 0.5, correspondingly. Among those women who participated in IDA testing and interview 68 were able to participate in both surveys.

Data collection

To evaluate the influence of the communication campaign participating women were interviewed by trained nurses at local health centres. The questionnaire included such topics as awareness on the health problems due to IDA, diets to prevent IDA and knowledge of healthy nutrition and fortified wheat flour.

The nurses also took blood samples from the participants. Hemoglobin (Hb) concentration of the capillary blood of the second drop of blood, being obtained by a puncture of a finger tip, using the HemoCue (HemoCue AB, Angelholm, Sweden) portable spectrophotometer. The venous blood sample was centrifuged and the separated serum was transported to the measure centre in frozen state, and serum ferritin (SF) was measured by the radio immune assay (Diagnostic Products Corporation, Los Angeles, CA) method. To define anemia and iron deficiency we used WHO cut-off criteria for developing countries [12,13]. Table 1 shows the definitions of anemia according to age group, applied for this study. The assessment of iron deficiency was performed through a measurement of SF. Cut off points for low level of ferritin or iron depletion was <12 μg/L for children under 5 years and <15 μg/L for children ≥ 5 years of age and women. IDA was defined in this study as those with anemia described in Table 1 and SF < 12 or 15 μg/L depend on age. Since 68 women participated in both surveys, their data were linked and the differences between the two surveys were compared.

Table 1. Definition of anemia according to blood hemoglobin (Hb) concentration (g/dl)

Statistical Analysis

Mean ± standard deviations were calculated for Hb and SF. The means of Hb and SF were examined with a paired t-test. Chi-square test and Fisher's exact test (in case of subjects <10) were also used to compare the distributions of categorical data. Two sided P-values < 0.05 were considered as significant. Statistical analyses were conducted using SPSS for Windows Version 11.5 (SPSS, Chicago, IL, USA).

Results

Hematological indices

Table 2 shows the percentages of subjects with anemia. Significant improvement in the prevalence of anemia between two surveys was observed for the women in the rural area (p < 0.05), and the children in the urban area (p < 0.001). The improvement in the prevalence of anemia was significant (p < 0.001) for the children in urban area. The data shows that it was also significant in the urban and rural areas combined. The prevalence of iron deficiency and IDA is shown in Table 3. The iron deficiency prevalence was significantly reduced among the children in urban, rural, and combined areas. The prevalence of IDA was significantly decreased among women in urban, urban and rural combined areas and urban children.

Table 2. Percentages and their 95% confidence intervals (CI) of subjects with anemia in the first (March) and second (December) surveys according to survey area

Table 3. Percentage and 95% CI of women and children with iron deficiency and iron deficiency anemia in the first (March) and second (December) surveys in urban and rural areas

Table 4 presents the average (Hb), geometric mean (SF) and standard deviation among 68 women who participated in both surveys. While the improvement in average Hb was significant among rural women, it was not significant among urban women. On the contrary, geometric mean of SF was significantly improved among the urban women. Figure 1 shows Hb distribution for all women before (March) and after (December) the campaign, the distribution for the survey on December was slightly shifted to the left compared with that of March.

Table 4. Hemoglobin (Hb) and serum ferritin (SF) levels of women in both surveys in urban and rural areas

thumbnailFigure 1. Distribution of blood hemoglobin for women aged 15-49 years before (March, N = 121) and after (December, N = 114) the campaign.

Questionnaire survey

As shown in Table 5, even in the first survey almost all of the women had heard about IDA. Among them more than 60.0% of both rural and urban women have chosen to eat iron rich foods and to take iron tablets as prevention against IDA. About 20.0% of women have chosen such answers as to go to sport and keep day regime (Figure 2). In the question about products rich in iron, the majority of women chose apple, meat, and liver, which are truly iron-rich products. Some of them chose eggs, potato, pumpkin and onion that contain less bioavailable iron or small amount of iron; the other of them chose dairy products and red beans that are even inhibiting iron absorption (Figure 3). About the knowledge on fortified wheat flour, a significant improvement was observed both among urban and rural women (Table 5).

Table 5. Percentage and 95% CI of women who answered, "yes" to the questions listed on the footnote

thumbnailFigure 2. Responses to the question, "How to prevent iron deficiency anemia?" (%). * Day regime doesn't include diet.

thumbnailFigure 3. Percentage of subjects who selected the item as iron-rich product for the question, "What products contain iron?". * Apple, liver, and meat are iron-rich foods.

The sources of information on IDA were assessed in two questions: generally (Table 6) and specifically through visual tools (Table 7). More significant sources of information were medical workers, TV, radio, and special brochures among urban women, and special brochures and medical posters among rural women (Table 6). Medical workers were marginally significant sources of information among rural women (p < 0.09). As shown in Table 7, the roles of TV, medical posters, posters in shops, and trademarks became significantly larger in the urban area, and TV and medical posters did in the rural area.

Table 6. Sources of information about iron deficiency anemia

Table 7. Visually received information sources of two surveys of the study

Discussion

In March, the prevalence of anemia was high (52.0% among the women and 58.1% among the children), and those with iron deficiency and IDA were prevalent (63.6%, 51.1% for iron deficiency and 40.5%, 11.0% for IDA, respectively). In December the prevalence of anemia was significantly reduced from 65.9% to 48.0% among rural women, and from 63.1% to 11.5% among urban children. The dramatic change in Hb status of urban children and the slight improvement without any behavioral change in rural women can be explained by intake of variety of fruits and vegetables taken during summer and autumn seasons.

The percentage for the iron deficiency among the children was significantly reduced from 51.1% to 24.8%. Significant improvement on IDA prevalence and some improvement in iron deficiency prevalence were observed among women in urban and urban and rural combined areas that can most possibly be due to taking iron pills after education campaign. Although the effects of the campaign on IDA were not consistent across the subgroups, they were unexpectedly large. The surveys found that most of women knew about IDA and that these numbers did not differ significantly in March and December. However, the prevalence of anemia, iron deficiency and IDA remained high among women. The main reasons for that are irrational nourishment, a strong tradition of drinking tea (high content of tannins in the tea) during meals, high birth rates, low levels of education, leading to lack of a culture of nourishment and habits of healthy nutrition [4,5]. These factors are not susceptible to change over a short time period.

Knowledge about fortified wheat flour among the women improved significantly both in urban (from 48.5% to 80.9%) and rural (from 69.8% to 88.6%) areas.

Food fortification is one of the most cost-effective and sustainable strategies for increasing iron intake in general populations [14-16]. Several trials have found that the consumption of iron-fortified staple foods or condiments improves iron status. The fortified products were iron-fortified fish sauce [17], salt fortified with microencapsulated iron, iodine, and vitamin A [18], iron-fortified curry powder [19], iron-fortified soy sauce [20], iron-fortified milk and drinking water [21], and sugar fortified with vitamin A and iron [22]. Forty-eight countries are currently enacting fortification programmes through voluntary or mandatory legislation. Twenty-eight of these countries fortify with iron and folic acid. Iron fortified flours have been used in different countries of the world including China, Venezuela and other American countries [23-26]. In Kazakhstan flour of the highest and first types was selected as the quality food vehicle, since it practically was present on each meal table, independent of family income. National food is based on the use of these types of flour. With the grinding of wheat flour almost 90% of all micronutrients are lost. The enrichment of wheat flour makes it possible to restore that loss.

Concerning the effects of the communication campaign on public awareness about the problems of anemia and importance of its prevention using fortified foods, however, there was limited information on the effectiveness. This study added findings on the effects of the campaign.

Several drawbacks of this study should be pointed out. Since the two surveys of the present study were made in March and December, that could influence the improvement on anemia and IDA prevalence and iron storage. We assume that March was after the period when the vegetables and fruits were relatively expensive, while December was after the period when vegetables and fruits were cheap and available. Secondly, whilst the effects on Hb and SF were examined with the subjects sampled before and after the campaign, not all of the subjects were the same for the March and December studies, so that the evidence produced must be considered indirect. However, improvement was observed among the 68 women who participated in both studies. Thirdly, SF might not reflect iron storage precisely. SF is an acute-phase reactant protein, which is elevated in response to infection. The underlying infectious diseases could mask the iron shortage among superficially healthy subjects [27]. However in our study we used the SF level indicator because its determination was widely used as a test to diagnose IDA. Iron stores decreases before serum iron levels, erythrocytes and Hb level decrease in the course of IDA. SF level is the best indicator for the women and children iron storage status and it is more sensitive and reliable than the other parameters.

Our results indicated unexplained inconsistency in Hb and SF levels between urban and rural women. Most likely this inconsistency is related to differential food access and approaches used by subjects to prevent IDA. Although health education had an influence on reduction of IDA and increases in iron stores in women, the positive effect on iron status will be temporary if these women's diets do not contain adequate bioavailable iron. And in this regard, WFF implementation will be very timely and helpful for sustainable improvement on anemia in the pilot region.

Conclusion

The study demonstrated that the communication campaign was effectively carried out in Kazakhstan before implementation of the WFF program, giving a biological impact on hematological indices.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

All authors contributed to study design. HN, NA, IK, and JS have been involved in drafting the manuscript, revising it critically for important intellectual content, and have given final approval of the version to be published. NA conceived of the study, performed statistical analysis, and interpreted analyzed data. NK and TI coordinated the study, and contributed to acquisition of data. BA is principal author of the paper, had full access to all data, and is guarantor. All authors contributed to manuscript drafting and revision and approved the final manuscript.

References

  1. Izmukhambetov T: Iron deficiency anaemia and health of the population of Kazakhstan. In Iron deficiency anaemia as regional problem in Kazakhstan: Epidemiological and nutritional aspects. Alma-Ata, National Institute of Nutrition; 1990:3-9. OpenURL

  2. Morse C: A study of the prevalence and causes of anemia, Muynak District, Karakalpakistan, the Republic of Uzbekistan. In Impact Food Security and Nutrition Monitoring Project. Washington, DC, USAID; 1994. OpenURL

  3. Preliminary report of a survey on anaemia in the Kzyl Orda region of Kazakhstan by the London Institute of Tropical Diseases In IMPACT Project. Washington, DC, USAID; 1994. OpenURL

  4. National Institute of Nutrition [Kazakstan], Academy of Preventive Medicine [Kazakstan], Macro International Inc: Kazakhstan Demographic and Health Survey, 1995. National Institute of Nutrition/Macro International Inc Calverton, MD; 1996.

  5. National Institute of Nutrition [Kazakstan], Academy of Preventive Medicine [Kazakstan], Macro International Inc: Kazakhstan Demographic and Health Survey, 1999. National Institute of Nutrition/Macro International Inc Calverton, MD; 2000.

  6. Sharmanov A: Anaemia in Central Asia: DHS Experience.

    Food and Nutrition Bulletin 1998, 19:307-317. OpenURL

  7. Dangour AD, Hill HL, Ismail SJ: Haemoglobin status of adult non-pregnant Kazakh women living in Kzyl-Orda region, Kazakhstan.

    Eur J Clin Nutr 2001, 55:1068-1075. PubMed Abstract | Publisher Full Text OpenURL

  8. Dangour AD, Hill HL, Ismail SJ: Height, weight and haemoglobin status of 6 to 59-month-old Kazakh children living in Kzyl-Orda region, Kazakhstan.

    Eur J Clin Nutr 2002, 56:1030-1038. PubMed Abstract | Publisher Full Text OpenURL

  9. Hashizume M, Kunii O, Sasaki S, Shimoda T, Wakai S, Mazhitova Z, Dauletbaev D, Caypil W, Aldiyarova M, Farmer A, Yamashiro Y, Chiba M: Anemia and iron deficiency among schoolchildren in the Aral Sea region, Kazakhstan.

    J Trop Pediatr 2003, 49:172-177. PubMed Abstract | Publisher Full Text OpenURL

  10. Hashizume M, Shimoda T, Sasaki S, Kunii O, Caypil W, Dauletbaev D, Chiba M: Anaemia in relation to low bioavailability of dietary iron among school-aged children in the Aral Sea region, Kazakhstan.

    Int J Food Sci Nutr 2004, 55:37-43. PubMed Abstract | Publisher Full Text OpenURL

  11. Hashizume M, Chiba M, Shinohara A, Iwabuchi S, Sasaki S, Shimoda T, Kunii O, Caypil W, Dauletbaev D, Alnazarova A: Anaemia, iron deficiency and vitamin A status among school-aged children in rural Kazakhstan.

    Public Health Nutr 2005, 8:564-571. PubMed Abstract | Publisher Full Text OpenURL

  12. Methods of assessing iron status In Iron Deficiency Anemia. Assessment, Prevention and Control. A Guide for programme managers. UNICEF/UNU/WHO; 2001:33-46. OpenURL

  13. Assessing the iron status of populations

    Assessment of Iron Status at the Population Level Report of a Joint WHO/CDC Technical Consultation Geneva, Switzerland 2004, 30. OpenURL

  14. Hurrell RF: Preventing iron deficiency through food fortification.

    Nutr Rev 1997, 55:210-222. PubMed Abstract OpenURL

  15. Lotfi M, Vencatesh Mannar MG, Merx JHMR, Heuvel PN: Micronutrient fortification of foods: current practices, research, and opportunities. Ottawa; Micronutrient Initiative/International Development Research Centre/International Agricultural Centre; 1996.

  16. Baltussen R, Knai C, Sharan M: Iron fortification and supplementation are cost-effective interventions to reduce iron deficiency in four subregions of the world.

    J Nutr 2004, 134:2678-2684. PubMed Abstract | Publisher Full Text OpenURL

  17. Thuy PV, Berger J, Davidsson L, Khan NC, Lam NT, Cook JD, Hurrell RF, Khoi HH: Regular consumption of NaFeEDTA-fortified fish sauce improves iron status and reduces the prevalence of anemia in anemic Vietnamese women.

    Am J Clin Nutr 2003, 78:284-90. PubMed Abstract | Publisher Full Text OpenURL

  18. Zimmermann MB, Wegmueller R, Zeder C, Chaouki N, Biebinger R, Hurrell RF, Windhab E: Triple fortification of salt with microcapsules of iodine, iron, and vitamin A.

    Am J Clin Nutr 2004, 80:1283-90. PubMed Abstract | Publisher Full Text OpenURL

  19. Ballot D, MacPhail A, Bothwell T, Gillooly M, Mayet FG: Fortification of curry powder with NaFe(111)EDTA in an iron-deficient population: report of a controlled iron-fortification trial.

    Am J Clin Nutr 1989, 49:162-9. PubMed Abstract | Publisher Full Text OpenURL

  20. Mannar V, Gallego E: Iron fortification: country level experiences and lessons learned.

    J Nutr 2002, 132:856S-8S. PubMed Abstract | Publisher Full Text OpenURL

  21. Beinner MA, Lamounier JA: Recent experience with fortification of foods and beverages with iron for the control of iron-deficiency anemia in Brazilian children.

    Food Nutr Bull 2003, 24:268-74. PubMed Abstract OpenURL

  22. Viteri FE, Alvarez E, Batres R, Torun B, Pineda O, Mejia LA, Sylvi J: Fortification of sugar with iron sodium ethylenediaminotetraacetate (FeNaEDTA) improves iron status in semirural Guatemalan populations.

    Am J Clin Nutr 1995, 61:1153-63. PubMed Abstract | Publisher Full Text OpenURL

  23. Nystrom J: Flour fortification database. Micronutrient Initiative, CDC; 2003.

  24. Sun J, Huang J, Li W, et al.: Effects of wheat flour fortified with different iron fortificants on iron status and anemia prevalence in iron-deficient anemic students in Northern China.

    Asia Pac J Clin Nutr 2007, 16:116-21. PubMed Abstract OpenURL

  25. Garcia-Casal MN, Layrisse M: Iron fortification of flours in Venezuela.

    Nutr Rev 2002, 60:S26-S29. PubMed Abstract | Publisher Full Text OpenURL

  26. Darnton-Hill I, Mora JO, Weinstein H, Wilbur S, Nalubola PR: Iron and folate fortification in the Americas to prevent and control micronutrient malnutrition: an analysis.

    Nutr Rev 1999, 57:25-31. PubMed Abstract OpenURL

  27. Hulthen L, Lindstedt G, Lundberg PA, Hallberg L: Effect of a mild infection on serum ferritin concentration-clinical and epidemiological implications.

    Eur J Clin Nutr 1998, 52:376-9. PubMed Abstract | Publisher Full Text OpenURL

Pre-publication history

The pre-publication history for this paper can be accessed here:

http://www.biomedcentral.com/1471-2326/10/2/prepub