Table 1

Murine specific primer sequences used in PCR

mRNA

Forward Primer

Reverse Primer

Expected product

size (bp)

GenBank accession

number


IL-1β

5'-gtgtgatgttcccattagacagc

5'-tgagaggtgctgatgtaccagtt

383

IL-6

5'-gaaaatctgctctggtcttctgg

5'-ttttctgaccacagtgaggaatg

357

M26744

IL-10

5'-tcagtcaagtgaagactttctttc

5'-gcagttgatgaagatgtcaaactc

358

X60675

TNFα

5'-tgaacttcggggtgatcggtc

5'-agccttgtcccttgaagagaac

295

X66539

KC (CXCL1)

5'-ttgaaggtgatgccgccag

5'-cccagactctcatctctcc

205

RANTES (CCL5)

5'-ccccatatggctcggacacca

5'-ctagctcatctccaaatagttgat

205

MCP-1 (CCL2)

5'-cactatgcaggtctctgtcacg

5'-gatctcacttggttctggtcca

294

M57441

MIP-2 (CXCL2)

5'-cagtgagctgcgctgtccaatg

5'-cagttagccttgcctttgttcag

210

X65647

GAPDH

5'-atgtcagatccacaacggatacat

5'-actccctcaagattgtcagcaat

307

ARP

5'-gttgaacatctcccccttctc

5'-atgtcctcatcggattcctcc

402

Z29530


Savard et al. BMC Gastroenterology 2002 2:23   doi:10.1186/1471-230X-2-23

Open Data