RT-PCR for cytokine mRNAs Treated MGBE were exposed to 100 μg/mL E. coli LPS on the apical surface for two hours. GAPDH was used as an internal control to standardize starting mRNA quantity. This experiment was repeated twice with equivalent results.
Savard et al. BMC Gastroenterology 2002 2:23 doi:10.1186/1471-230X-2-23