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Open Access Research article

Up-regulation of CNDP2 facilitates the proliferation of colon cancer

Conglong Xue1, Zhenwei Zhang2, Honglan Yu1, Miao Yu1, Kaitao Yuan1, Ting Yang3, Mingyong Miao2* and Hanping Shi1*

Author Affiliations

1 Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan II Road, Guangzhou, Guangdong 510080, China

2 Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China

3 Department of Intensive Care Unit, Zhongshan people’s Hospital, 2 Sunwen East Road, Zhongshan, Guangdong 528403, China

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BMC Gastroenterology 2014, 14:96  doi:10.1186/1471-230X-14-96

Published: 21 May 2014

Abstract

Background

Cytosolic nonspecific dipetidase (CN2) belongs to the family of M20 metallopeptidases. It was stated in previous articles that higher expression levels of CN2 were observed in renal cell carcinoma and breast cancer. Our study explored the correlation between CN2 and colon carcinogenesis.

Methods

We analysed the relationship between 183 patients clinicopathological characteristics and its CN2 expression. To detect the levels of CN2 in colon cancer cell lines and colon cancer tissues by western blot. To verify cell proliferation in colon cancer cells with knockdown of CNDP2 and explore the causes of these phenomena.

Results

The expression levels of CN2 in clinical colon tumors and colon cancer cell lines were significantly higher than that in normal colon mucosa and colon cell lines. The difference in CN2 levels was associated with tumor location (right- and left-sided colon cancer), but there was no significant association with age, gender, tumor size, tumor grade, tumor stage or serum carcinoembryonic antigen (CEA). Knockdown of CNDP2 inhibited cell proliferation, blocked cell cycle progression and retarded carcinogenesis in an animal model. The signaling pathway through which knockdown of CNDP2 inhibited cell proliferation and tumorigenesis involved in EGFR, cyclin B1 and cyclin E.

Conclusions

Knockdown of CNDP2 can inhibit the proliferation of colon cancer in vitro and retarded carcinogenesis in vivo.

Keywords:
CNDP2; Colon cancer; Poliferation; Clinicopathological Characteristics; RNA interference