Deregulated expression of circadian clock genes in gastric cancer
- Equal contributors
1 Division of Hepatogastroenterology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 123 Da-Pei Road, Niaosung District, 833 Kaohsiung, Taiwan
2 Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road,Kwei-Shan 333 Tao-Yuan, Taiwan
3 Department of Pathology, Changhua Christian Hospital, 135 Nan-Hsiao St., 500 Changhua, Taiwan
4 Department of Nursing, I-Shou University, No.1, Sec. 1, Syuecheng Road, Dashu District, 840 Kaohsiung City, Taiwan
5 Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 123 Da-Pei Road, Niaosung District, 833 Kaohsiung City, Taiwan
6 Division of Hematology-Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, 807 Kaohsiung City, Taiwan
7 Faculty of Medicine, Kaohsiung Medical University, 100 Tzyou 1st Road, 807 Kaohsiung City, Taiwan
8 Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, 807 Kaohsiung, Taiwan
9 Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, 68 Jhonghua 3rd Road, 801 Kaohsiung, Taiwan
BMC Gastroenterology 2014, 14:67 doi:10.1186/1471-230X-14-67Published: 6 April 2014
Gastric cancer (GC), an aggressive malignant tumor of the alimentary tract, is a leading cause of cancer-related death. Circadian rhythm exhibits a 24-hour variation in physiological processes and behavior, such as hormone levels, metabolism, gene expression, sleep and wakefulness, and appetite. Disruption of circadian rhythm has been associated with various cancers, including chronic myeloid leukemia, head and neck squamous cell carcinoma, hepatocellular carcinoma, endometrial carcinoma, and breast cancer. However, the expression of circadian clock genes in GC remains unexplored.
In this study, the expression profiles of eight circadian clock genes (PER1, PER2, PER3, CRY1, CRY2, CKIϵ, CLOCK, and BMAL1) of cancerous and noncancerous tissues from 29 GC patients were investigated using real-time quantitative reverse-transcriptase polymerase chain reaction and validated through immunohistochemical analysis.
We found that PER2 was significantly up-regulated in cancer tissues (p < 0.005). Up-regulated CRY1 expression was significantly correlated with more advanced stages (stage III and IV) (p < 0.05).
Our results suggest deregulated expressions of circadian clock genes exist in GC and circadian rhythm disturbance may be associated with the development of GC.