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Open Access Research article

MiR-195 affects cell migration and cell proliferation by down-regulating DIEXF in Hirschsprung’s Disease

Hao Lei13, Junwei Tang13, Hongxing Li13, Hongwei Zhang4, Changgui Lu13, Huan Chen13, Wei Li13, Yankai Xia12 and Weibing Tang13*

  • * Corresponding author: Weibing Tang twbcn@163.com

  • † Equal contributors

Author Affiliations

1 State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China

2 Key Laboratory of Modern Toxicology (Nanjing Medical University), Ministry of Education, Nanjing, China

3 Department of Pediatric Surgery, Nanjing Children’s Hospital Affiliated Nanjing Medical University, Nanjing 210008, China

4 Department of Pediatric Surgery, Xuzhou Children’s Hospital Affiliated Xuzhou Medical University, Xuzhou 221002, China

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BMC Gastroenterology 2014, 14:123  doi:10.1186/1471-230X-14-123

Published: 9 July 2014

Abstract

Background

Hirschsprung’s disease (HSCR) is the most common congenital gut motility disorder. We aimed to investigate the roles of miR-195 in the pathogenesis of HSCR.

Methods

In this study, we measured the expression levels of miRNA, mRNA, and protein in colon tissues from 78 patients with HSCR and 66 controls without HSCR. Transwell, Cell Counting Kit-8 (CCK-8) and flow cytometry assay were employed to detect the function role of miR-195 in vitro.

Results

Our results showed that expression levels of miR-195 from patients with HSCR were significantly higher than control group; along with aberrant lower expression levels of digestive-organ expansion factor (DIEXF) were tested. Increased level of miR-195 could suppress the level of DIEXF in cell, which induced the impairment of cell migration and proliferation.

Conclusions

Aberrant expression of miR-195 may involved in the pathogenesis of HSCR by down-regulated the level of DIEXF.

Keywords:
microRNA; Congenital diseases; Migration; Gastroenterology; HSCR