Time-dependent changes in liver respiration in ConA hepatitis in C57Bl/6 IFN-γ−/−mice. Mice were injected with 20 mg ConA/kg and liver specimens (24–29 mg) were collected for O2 measurements at 3, 6, and 12 hr post-injection. Control mice were injected with PBS. Rates of respiration (kc, in μM O2 min-1 · mg-1) are shown at the bottom of the runs. All four runs were performed on one instrument. One animal was sacrificed at-a-time in the following sequence: 12 hr post-injection, 3 hr post-injection, PBS, and then 6 hr post-injection. Minute zero corresponds to sacrifice of the first animal.
Al-Shamsi et al. BMC Gastroenterology 2013 13:6 doi:10.1186/1471-230X-13-6