The composition of commensal bacteria is altered in naive GC-C−/− mice relative to GC-C+/+. Real time PCR of bacterial genomic DNA extracted from stool was performed using family-specific bacterial 16S rRNA gene primers. There was significant enrichment of Enterobacteriaceae and Atopobium in GC-C−/− compared to GC-C+/+ while the level of Lactobacillus was decreased. Line represents median value. (Entero., Enterobacteriaceae; Lacto., Lactobacillus; Bifido., Bifidobacteria; E. rectale, Eubacterium rectale; C. cocoides, Clostridiae cocoides; WT, wildtype GC-C+/+; KO, GC-C−/−; *P < 0.03, ** P < 0.005, n = 10–12 per group).
Mann et al. BMC Gastroenterology 2013 13:135 doi:10.1186/1471-230X-13-135