Additional file 8.
Figure S8.Deletion of Foxp3+Treg worsens acute intestinal inflammation. (A) IFN-γ and IL-17A production of PBS-treated and DTx-treated Foxp3-GFP-DTR mice and DTx-treated Foxp3-GFP mice. DTx administered on day-1, day 1, and day 3. CD4+ cells were extracted from mesentric lymph nodes and stimulated for 48h. Cytokine concentrations were determined in culture supernatants by ELISA. Data shown are the tabular representation of the mean values ± SD of the concentration of the distinct secreted cytokines derived from five mice per group each analyzed in duplicate. Statistical significance was analyzed by two-tailed unpaired student’s t-test with a 95% confidence interval, *,p≤0.05. (B) Comparison of the IFN-γ and IL-17A production of CD4+ cells isolated from Foxp3-GFP-DTR mice treated with DTx versus the two different control groups. Data shown are the corresponding p values analyzed by a two-tailed unpaired student’s t-test with a 95% confidence interval.
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Boehm et al. BMC Gastroenterology 2012 12:97 doi:10.1186/1471-230X-12-97