Figure 5.

Deletion of Foxp3+Treg worsens acute intestinal inflammation. (A) Body weight as a percent of starting weight of PBS-treated and DTx-treated Foxp3-GFP-DTR mice and DTx-treated Foxp3-GFP mice. DTx was administered on day −1,day 1, and day 3. (B) Survival of PBS-treated and DTx-treated Foxp3-GFP-DTR mice and DTx-treated Foxp3-GFP mice. DTx was administered on day −1,day 1, and day 3. (C) H&E staining and histology score of representative colon sections of PBS-treated and DTx-treated Foxp3-GFP-DTR mice and DTx-treated Foxp3-GFP mice. DTx was administered on day −1,day 1, and day 3. Horizontal lines of the scatter plot are mean values derived from five mice per group. The error bars represent the SEM. Each point represents the histological score of the corresponding mouse. Statistical significance was analyzed by two-tailed unpaired student´s t-test with a 95% confidence interval. *, p ≤0.05. (D) IFN-γ and IL-17A production of PBS-treated and DTx-treated Foxp3-GFP-DTR mice and DTx-treated Foxp3-GFP mice. DTx was administered on day −1,day 1, and day 3. CD4+ cells were extracted from mesenteric lymph nodes and stimulated for 48 h. Cytokine concentrations were determined in culture supernatants by ELISA. Data shown are mean values ± SD and derived from five mice per group each analyzed induplicate. Statistical significance was analyzed by two-tailed unpaired student´s t-test with a 95% confidence interval. *, p ≤0.05.

Boehm et al. BMC Gastroenterology 2012 12:97   doi:10.1186/1471-230X-12-97
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