Figure 3.

Deletion of Foxp3+Treg activates CD4+T cells to produce cytokines. (A) IFN-γ, IL-13, and IL-17A production after 10 days of Foxp3+ Treg deletion. CD4+ cells were extracted from mesenteric lymph nodes and stimulated for 48h. Cytokine concentrations were determined in culture supernatants by ELISA. Data shown are mean values ± SD and derived from five mice per group each analyzed induplicate. Statistical significance was analyzed by two-tailed unpaired student´s t-test with a 95% confidence interval. *, p ≤0.05. (B) IFN-γ, IL-13, and IL-17A production after 10 days of Foxp3+ Treg deletion. CD4+ cells were extracted from the spleen and stimulated for 48 h. Cytokine concentrations were determined in culture supernatants by ELISA. Data shown are mean values ± SD and derived from five mice per group each analyzed induplicate. Statistical significance was analyzed by two-tailed unpaired student´s t-test with a 95% confidence interval. *, p ≤0.05.

Boehm et al. BMC Gastroenterology 2012 12:97   doi:10.1186/1471-230X-12-97
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