CD4+CD25-cells up-regulate markers associated with regulatory phenotype. TregPMA cells express the markers CTLA-4 (A), GARP (B), ICOS (C) and OX40 (D) associated with a regulatory phenotype and function. Representative histogram overlays are shown. In red CD4+CD25- T cells at time 0 and in blue TregPMA cells after following PMA/ionomycin/anti-CD3 activation and 5 days in culture.
Majowicz et al. BMC Gastroenterology 2012 12:172 doi:10.1186/1471-230X-12-172