Open Access Research article

Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract

Luca Badiali12, Jonathan Cedernaes1, Pawel K Olszewski13, Olof Nylander1, Anna V Vergoni2 and Helgi B Schiöth1*

Author Affiliations

1 Department of Neuroscience, Uppsala University, BMC, Uppsala, SE 75124, Sweden

2 Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi, 41125, Modena, Italy

3 Department of Biological Sciences, University of Waikato, Hamilton, New Zealand

For all author emails, please log on.

BMC Gastroenterology 2012, 12:134  doi:10.1186/1471-230X-12-134

Published: 25 September 2012

Additional files

Additional file 1:

Primers used for real-time PCR analysis. The table includes gene names for rat (r) Adhesion GPCRs and house-keeping genes (*), GenBank accession numbers, primer sequences and the expected size of the PCR products . NA – not available.

Format: PDF Size: 12KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Summary of relative expression of all Adhesion GPCR members. Expression levels relative for each gene (maximal level of expression set to 1; n.d., not detected after 40 cycles of the PCR). The phylogenetic grouping is based on the 7TM regions [3]. GPR111, GPR113, GPR115 were only expressed in one sub-segment: the expression value was arbitrarily set to 1 to indicate the presence of expression. GPR133 and CELSR3 could not be detected in any segment.

Format: PDF Size: 25KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data