Sphere-forming cells overexpressed liver CSC related proteins and downstream proteins of the CSL-independent Notch pathway. (A) The PLC/PRF/5 sphere-forming cells expressed the hepatic stem cell maker OV6, as observed under fluorescence microscopy. Nuclei were stained with DAPI (400×). (B) Western blotting assay showed the liver CSC related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44) and the relative band intensities were calculated by densitometry and normalized to the loading control GAPDH.
Cao et al. BMC Gastroenterology 2011 11:71 doi:10.1186/1471-230X-11-71