Hepatoma cells formed the anchorage-independent, self-renewing spheres. (A) Hepatoma cell lines, including HepG2, MHCC97H and PLC/PRF/5, could form the anchorage-independent 3-D spheres in stem cell conditioned culture medium (200×). (B) Generation of a sphere from a single PLC/PRF/5 cell. The propagation of a single cell cultured in a 96-well dish was recorded at day 1, 3, 5, 7, 9 and 13, separately (400×).
Cao et al. BMC Gastroenterology 2011 11:71 doi:10.1186/1471-230X-11-71