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Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines

Lu Cao1, Yanming Zhou2, Beibei Zhai1, Jian Liao1, Wen Xu1, Ruixiu Zhang1, Jing Li1, Yu Zhang1, Lei Chen1, Haihua Qian1, Mengchao Wu1 and Zhengfeng Yin1*

Author Affiliations

1 Department of Molecular Oncology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China

2 Department of Hepato-Biliary-Pancreato-Vascular Surgery, the First affiliated Hospital of Xiamen University, Xiamen, China

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BMC Gastroenterology 2011, 11:71  doi:10.1186/1471-230X-11-71

Published: 14 June 2011

Additional files

Additional file 1:

Figure S1. Tumorigenic efficacies of the hepatoma sphere-forming cells. The hepatoma parental cells and sphere-forming cells were injected into nude mice subcutaneously at the indicated cell concentrations. At day 30th after injection, mice were sacrificed and tumors were removed and compared in size.

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Additional file 2:

Figure S2. Expression of candidate CSC markers in hepatoma sphere-forming cells. Confocal immunofluorescent staining showed that CD44 expression was enriched obviously in HepG2 and MHCC97H sphere-forming cells compared with their parental cells. Nuclei were stained with DAPI (400×).

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