Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
1 Department of Molecular Oncology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China
2 Department of Hepato-Biliary-Pancreato-Vascular Surgery, the First affiliated Hospital of Xiamen University, Xiamen, China
BMC Gastroenterology 2011, 11:71 doi:10.1186/1471-230X-11-71Published: 14 June 2011
Additional file 1:
Figure S1. Tumorigenic efficacies of the hepatoma sphere-forming cells. The hepatoma parental cells and sphere-forming cells were injected into nude mice subcutaneously at the indicated cell concentrations. At day 30th after injection, mice were sacrificed and tumors were removed and compared in size.
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Additional file 2:
Figure S2. Expression of candidate CSC markers in hepatoma sphere-forming cells. Confocal immunofluorescent staining showed that CD44 expression was enriched obviously in HepG2 and MHCC97H sphere-forming cells compared with their parental cells. Nuclei were stained with DAPI (400×).
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