Fucoidan reduces the viability of HT-29 and HCT116 cells. HT-29 (A), HCT116 (B) and FHC (C) cells were plated in 24-well plates at a density of 50,000 cells/well with DMEM/F12 supplemented with 10% FBS. One day later, the monolayers were serum-deprived with DMEM/F12 supplemented with 1% charcoal-stripped FBS (serum-deprivation medium) for 24 h. Following serum deprivation, the cells were incubated in serum-deprivation medium containing 0 - 20 μg/mL of fucoidan. Viable cell numbers were estimated via MTT assays. Each bar represents the mean ± SEM (n = 6). Means at a time without a common letter differ, P < 0.05.
Kim et al. BMC Gastroenterology 2010 10:96 doi:10.1186/1471-230X-10-96