Timing and extent of hepatocyte proliferation post-CCl4 is similar in both GC-C KO and WT. PCNA IHC was used to examine hepatocyte entry into the cell cycle. Both PCNA-labeled and unlabeled nuclei (counterstained with hematoxylin) were counted in 3 fields (200×) and the percentage of PCNA-positive nuclei was calculated (≥ 1100 total nuclei per mouse, N = ≥ 4 mice per time point).
Mann et al. BMC Gastroenterology 2010 10:86 doi:10.1186/1471-230X-10-86