Increased post-CCl4 hepatocyte apoptosis in GC-C null livers . (A) Representative photomicrographs of cleaved Caspase 3 antibody staining (brown color) in livers (counterstained with hematoxylin) from WT and GC-C KO mice at 2 days after CCl4 administration (200 × magnification). (B) Cleaved Caspase 3 stained cells were counted in 10 fields (200×) per mouse at the indicated times. Data is presented as the mean number ± SEM, N = ≥ 4 mice per time point. *P = 0.04, GC-C KO vs. WT, Day 2. (C) Caspase 3 activity was measured using the colorimetric substrate Ac-DEVD-pNA (N = ≥ 3mice per time point). Activity levels were normalized to the level seen in vehicle injected liver (CONT). *P = 0.03, GC-C KO vs. WT, Day 2.
Mann et al. BMC Gastroenterology 2010 10:86 doi:10.1186/1471-230X-10-86