Open Access Research article

Blood spots as an alternative to whole blood collection and the effect of a small monetary incentive to increase participation in genetic association studies

Parveen Bhatti12*, Diane Kampa3, Bruce H Alexander3, Christopher McClure4, Danny Ringer4, Michele M Doody2 and Alice J Sigurdson2

Author Affiliations

1 Program in Epidemiology, Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

2 Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, Bethesda, MD, USA

3 Division of Environmental Health Sciences, School of Public Health, University of Minnesota, Minneapolis, MN, USA

4 Research Triangle Institute, Rockville, MD, USA

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BMC Medical Research Methodology 2009, 9:76  doi:10.1186/1471-2288-9-76

Published: 13 November 2009

Abstract

Background

Collection of buccal cells from saliva for DNA extraction offers a less invasive and convenient alternative to venipuncture blood collection that may increase participation in genetic epidemiologic studies. However, dried blood spot collection, which is also a convenient method, offers a means of collecting peripheral blood samples from which analytes in addition to DNA can be obtained.

Methods

To determine if offering blood spot collection would increase participation in genetic epidemiologic studies, we conducted a study of collecting dried blood spot cards by mail from a sample of female cancer cases (n = 134) and controls (n = 256) who were previously selected for a breast cancer genetics study and declined to provide a venipuncture blood sample. Participants were also randomized to receive either a $2.00 bill or no incentive with the blood spot collection kits.

Results

The average time between the venipuncture sample refusal and recruitment for the blood spot collection was 4.4 years. Thirty-seven percent of cases and 28% of controls provided a dried blood spot card. While the incentive was not associated with participation among controls (29% for $2.00 incentive vs. 26% for no incentive, p = 0.6), it was significantly associated with participation among the breast cancer cases (48% vs. 27%, respectively, p = 0.01). There did not appear to be any bias in response since no differences between cases and controls and incentive groups were observed when examining several demographic, work history and radiation exposure variables.

Conclusion

This study demonstrates that collection of dried blood spot cards in addition to venipuncture blood samples may be a feasible method to increase participation in genetic case-control studies.