PAI-1 steady state levels in primary cultures of fibroblasts with several WRN genotypes. WRN C1367R and L1074F polymorphisms were determined by automatic sequencing in primary cultured fibroblasts obtained from healthy adult donors; steady state levels of PAI-1 were determined by semiquantitative RT-PCR after 26 amplification cycles. One representative agarose gel is shown in inset. Results were normalized to β-actin expression and plotted as mean ± standard deviation of arbitrary units. Number of cultures from each genotype is shown above each bar. * Groups used for ANOVA test • Groups with statistic differences against heterozygous carriers (1367CR/1704FL) by Tukey test, P < 0.01. M = molecular weight ladder.
Castro et al. BMC Cardiovascular Disorders 2008 8:5 doi:10.1186/1471-2261-8-5