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Open Access Highly Accessed Research article

Vitamin E and telmisartan attenuates doxorubicin induced cardiac injury in rat through down regulation of inflammatory response

Najah Hadi2, Nasser Ghaly Yousif1*, Fadhil G Al-amran2, Nadhem K Huntei2, Bassim I Mohammad3 and Sadiq J Ali2

Author Affiliations

1 University of Colorado Denver, department of Medicine and Surgery Aurora, Denver, CO, 80045, USA

2 Kufa University, College of medicine, Najaf, Iraq

3 Al-Qadesiyah college of Medicine, Al-Qadesiya, Iraq

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BMC Cardiovascular Disorders 2012, 12:63  doi:10.1186/1471-2261-12-63

Published: 6 August 2012

Abstract

Background

The importance of doxorubicin (Dox), as a potent antitumor antibiotic, is limited by the development of life-threatening cardiomyopathy. It has been shown that free radicals are involved in acute doxorubicin-induced toxicity. The aim of this study was to determine the protective effect of vitamin E and telmisartan in acute doxorubicin induced cardiotoxicity.

Methods

Thirty two male Sprague - Dawly rats were involved in this study and were randomly separated into 4 groups, eight rats in each group, one group received normal saline I.P as control and second group received doxorubicin 20 mg/kg I.P, the other two groups also received doxorubicin 20 mg/kg I.P as single dose after seven cumulative doses (for seven days) of vitamin E (100 mg/kg) and telmisartan (1 mg/kg) respectively. Immunofluorescent staining for monocytes infiltration and analyses of plasma by (ELISAs) for MCP-1and troponin I. Western immunoblotting assay for ICAM-1, while left ventricular function was analyzed by microcatheter, also estimated the level of oxidative stress parameters (MDA and Catalase) and cardiac enzymes activities (CK-MB and LDH) before starting drugs treatment and after treatment period by 48 hours.

Results

The immunofluorescent staining showed that administration of vitamin E and telmisartan are attenuated of mononuclear cell infiltration; (pā€‰<ā€‰0.05 vs. Dox group), also reduced the level of chemokines MCP-1 and ICAM-1 expression compared with Dox group only, and there is marked reduction of myocardial troponin-I levels with improved LV function in vitamin E and telmisartan treated group. Doxorubicin treatment increased MDA, LDH, CK-MB levels significantly (Pā€‰<ā€‰0.01), and were counteracted by administration of vitamin E and telmisartan, but did not significantly affect serum catalase activity.

Conclusions

Antioxidant effect (Vitamin E and telmisartan) have been shown to decrease doxorubicininduced cardiotoxicity.