Altered expression of microRNAs in the myocardium of rats with acute myocardial infarction
1 Department of Cardiology, Peking University Third Hospital, Beijing, China
2 Department of Cardiology, Beijing Military General Hospital, Beijing, China
3 MOE key Laboratory of Molecular Cardiology, Peking University, Beijing, China
4 Department of Biomedical Informatics, Peking University Health Science Center, Beijing, China
BMC Cardiovascular Disorders 2010, 10:11 doi:10.1186/1471-2261-10-11Published: 1 March 2010
MicroRNAs(miRNAs) are important cellular components and their dysfunction is associated with various diseases. Acute myocardial infarction (AMI) is one of the most serious cardiovascular diseases. Although several miRNAs are reported to be associated with AMI, more novel miRNAs are needed to further investigate and improve certainty
We applied a well-established acute myocardial infarction rat model and performed miRNAs microarray experiments upon the myocardium tissue of rats with AMI and under sham control. We identified the differentially expressed miRNAs and analyzed the function of miRNA targets, transcription factors, and host genes based on bioinformatics.
As a result, the levels of expression of seventeen miRNAs significantly deregulated, of which four miRNAs were further validated by qRT-PCR. In addition, we observed that the transcription factors, targets, and host genes of these deregulated miRNAs are enriched in cardiovascular-related functions.
We found that the miRNAs expression level altered in rats with AMI and differentially expressed miRNAs may be novel biomarkers of AMI.