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Open Access Highly Accessed Research article

Altered expression of microRNAs in the myocardium of rats with acute myocardial infarction

Bing Shi123, Yanhong Guo13, Juan Wang34* and Wei Gao13*

Author Affiliations

1 Department of Cardiology, Peking University Third Hospital, Beijing, China

2 Department of Cardiology, Beijing Military General Hospital, Beijing, China

3 MOE key Laboratory of Molecular Cardiology, Peking University, Beijing, China

4 Department of Biomedical Informatics, Peking University Health Science Center, Beijing, China

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BMC Cardiovascular Disorders 2010, 10:11  doi:10.1186/1471-2261-10-11

Published: 1 March 2010

Abstract

Background

MicroRNAs(miRNAs) are important cellular components and their dysfunction is associated with various diseases. Acute myocardial infarction (AMI) is one of the most serious cardiovascular diseases. Although several miRNAs are reported to be associated with AMI, more novel miRNAs are needed to further investigate and improve certainty

Methods

We applied a well-established acute myocardial infarction rat model and performed miRNAs microarray experiments upon the myocardium tissue of rats with AMI and under sham control. We identified the differentially expressed miRNAs and analyzed the function of miRNA targets, transcription factors, and host genes based on bioinformatics.

Results

As a result, the levels of expression of seventeen miRNAs significantly deregulated, of which four miRNAs were further validated by qRT-PCR. In addition, we observed that the transcription factors, targets, and host genes of these deregulated miRNAs are enriched in cardiovascular-related functions.

Conclusion

We found that the miRNAs expression level altered in rats with AMI and differentially expressed miRNAs may be novel biomarkers of AMI.