Table 2 |
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|
Markers used to survey population diversity* |
||||||
|
Marker name |
Reverse primer |
Forward primer (includes M13 tail) |
Repeat unit |
No. of Repeats |
No. of alleles in six Bd lines |
No. of alleles in all lines |
|
|
||||||
|
ALB001 |
ttctccaccacactccttcc |
cacgacgttgtaaaacgacgatcgtgttcttccccttca |
ct |
22 |
4 |
12 |
|
ALB006 |
cctcctccaacaaccacagt |
cacgacgttgtaaaacgactagtagcccccaagccttct |
gt |
15 |
4 |
10 |
|
ALB008 |
atgggcgagaagacagagaa |
cacgacgttgtaaaacgacacgggaactcaccatttcac |
ctt |
13 |
4 |
11 |
|
ALB013 |
atccgtgtttcgttctttgg |
cacgacgttgtaaaacgactttgccaatgcttcaaactg |
ga |
12 |
3 |
9 |
|
ALB022 |
atttctgcccagcaaacact |
cacgacgttgtaaaacgacgacctcccctgtctcaggt |
ct |
11 |
3 |
12 |
|
ALB030 |
gtagtccagccccatcttcc |
cacgacgttgtaaaacgaccgtccgcaagcttgtttt |
ttg |
9 |
3 |
16 |
|
ALB034 |
tgctgcttcttttgtcgatg |
cacgacgttgtaaaacgacaatggtggatctgcagaagg |
aac |
9 |
3 |
7 |
|
ALB040 |
agtcctcctcctcgctaagg |
cacgacgttgtaaaacgacccctgcttccctctctctct |
ctt |
8 |
3 |
10 |
|
ALB050 |
ggagggagaaaaatgccttc |
cacgacgttgtaaaacgactagtagcccccaagccttct |
gt |
15 |
4 |
11 |
|
ALB056 |
tccacagagcaccacagaag |
cacgacgttgtaaaacgactcctctggttcctgagatcg |
gt |
10 |
3 |
9 |
|
ALB086 |
caactgatcccgagctcttc |
cacgacgttgtaaaacgacgtccggaaccaacgaaaac |
aag |
7 |
3 |
7 |
|
ALB087 |
gacttgatgaagccctgctc |
cacgacgttgtaaaacgacacaggcagcagcaggaac |
agc |
7 |
3 |
6 |
|
ALB089 |
tgagtcgaataagccggaag |
cacgacgttgtaaaacgaccttcacccagctgctcatc |
cag |
7 |
3 |
8 |
|
ALB100 |
caggtacgtcaccaggttca |
cacgacgttgtaaaacgaccggagacgacgacagagg |
gca |
7 |
3 |
14 |
|
ALB131 |
gacacatcgttggcaatgtc |
cacgacgttgtaaaacgaccaacggagtggtacgttgtg |
ggc |
7 |
4 |
7 |
|
ALB139 |
tgtaccggaggatgaagtcc |
cacgacgttgtaaaacgacgtgccaaatccaagaaggaa |
aga |
7 |
3 |
11 |
|
ALB155 |
atatcacccccacaggaacc |
cacgacgttgtaaaacgactgatcagagtccccaaaacc |
ct |
15 |
3 |
6 |
|
ALB158 |
tgtcttgtcccctgcgata |
cacgacgttgtaaaacgacgcgctccttgagctgtattg |
gaa |
14 |
4 |
14 |
|
ALB160 |
tgctgatcccatttcctcat |
cacgacgttgtaaaacgacgtgcccgaggactatcatgt |
ct |
13 |
3 |
10 |
|
ALB165 |
atttgccccacaaatggtta |
cacgacgttgtaaaacgacttcgtggttcaacaacatgg |
ata |
12 |
3 |
18 |
|
ALB170 |
agggtggctgtttagacgaa |
cacgacgttgtaaaacgacttgggaatctccaggttcag |
tg |
10 |
3 |
15 |
|
ALB175 |
ctgacgttagggtggctgtt |
cacgacgttgtaaaacgacttgggaatctccaggttcag |
tg |
10 |
4 |
9 |
|
ALB179 |
caccggaagtggagaagaag |
cacgacgttgtaaaacgactgatcaagtgcaaggaaacg |
ct |
9 |
4 |
24 |
|
ALB181 |
gggttccacctgtcagaaaa |
cacgacgttgtaaaacgacatgccaaatgggactgtttc |
ac |
9 |
3 |
6 |
|
ALB183 |
tggtgatttaaatggcacaaa |
cacgacgttgtaaaacgacgggatatgcccaattttgaa |
tct |
9 |
3 |
7 |
|
ALB223 |
aagacatccaaccgaccaag |
cacgacgttgtaaaacgacactgctgcgtttcgtcaga |
aat |
6 |
3 |
7 |
|
ALB230 |
taaaggggcaaattgcaaag |
cacgacgttgtaaaacgactcgcaatgaaaccctaggtc |
atct |
6 |
3 |
13 |
|
ALB257 |
tgtgatggattttgcttcca |
cacgacgttgtaaaacgactgtcgctcctgcatctattg |
aatt |
5 |
3 |
10 |
|
ALB273 |
tccatctccatcatcccttc |
cacgacgttgtaaaacgactggggaacatttccatcatt |
ga |
8 |
3 |
4 |
|
ALB278 |
cctgggttaattatggcctgt |
cacgacgttgtaaaacgaccgtatctattctcacctccgatg |
tc |
8 |
3 |
5 |
|
ALB311 |
cgtcgtcttcaggtctttcc |
cacgacgttgtaaaacgaccctaacagcttccgtctcca |
ga |
6 |
3 |
9 |
|
ALB348 |
tgtgtgccgaactagtgaaag |
cacgacgttgtaaaacgactccaggccctcacatatctc |
tc |
13 |
4 |
13 |
|
ALB349 |
ggatggctctcaaggtcact |
tcacgacgttgtaaaacgacgagcatgtgggtgtgattt |
tc |
13 |
5 |
16 |
|
ALB355 |
cagcaggtcctcgtactcct |
cacgacgttgtaaaacgacgatacatcccagccattaatcc |
ctt |
11 |
4 |
16 |
|
ALB372 |
ggtgcgcaatggagatagat |
cacgacgttgtaaaacgactctaggctccgttccgagta |
tatc |
9 |
4 |
13 |
|
ALB374 |
aatggtggatctgcagaagg |
cacgacgttgtaaaacgactgctgcttcttttgtcgatg |
gtt |
9 |
3 |
7 |
|
ALB376 |
gggttccacctgtcagaaaa |
cacgacgttgtaaaacgacatgccaaatgggactgtttc |
ac |
9 |
3 |
6 |
|
ALB445 |
caccagcgtttacgtagcag |
cacgacgttgtaaaacgactgttcagtgggtcgagtcaa |
gaaa |
7 |
3 |
11 |
|
ALB454 |
cttcacccagctgctcatc |
cacgacgttgtaaaacgactgagtcgaataagccggaag |
tgc |
7 |
3 |
9 |
|
ALB461 |
gggccgtctgcatttatcat |
cacgacgttgtaaaacgaccagaactgtcagtcccctttg |
at |
7 |
3 |
6 |
|
ALB467 |
ggcgtcacgaaagaagagaa |
cacgacgttgtaaaacgacccccaccagctctatgaaat |
at |
7 |
3 |
6 |
|
ALB486 |
ggccagccatgttagactgt |
cacgacgttgtaaaacgactaatccgcgtcctctcttgt |
ag |
6 |
3 |
3 |
|
ALB514 |
aaaagaaccccgacctgaat |
cacgacgttgtaaaacgacacggagggagtacaccacaa |
aag |
6 |
3 |
15 |
|
Avg = 3.3 |
Avg = 10.2 |
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|
|
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|
* PCR product sizes for all lines can be found in additional file 7. |
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|
Vogel et al. BMC Plant Biology 2009 9:88 doi:10.1186/1471-2229-9-88 |
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