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Open Access Research article

The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

Florian Schröder1, Janina Lisso1, Peggy Lange2 and Carsten Müssig3*

Author Affiliations

1 Max Planck Institute of Molecular Plant Physiology, Dept. Willmitzer, Am Mühlenberg 1, 14476 Potsdam – Golm, Germany

2 Universität Potsdam, Karl-Liebknecht-Str. 24/25, Haus 26, 14476 Potsdam – Golm, Germany

3 GoFORSYS, Universität Potsdam, c/o Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam – Golm, Germany

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BMC Plant Biology 2009, 9:20  doi:10.1186/1471-2229-9-20

Published: 13 February 2009

Abstract

Background

The EXO (EXORDIUM) gene was identified as a potential mediator of brassinosteroid (BR)-promoted growth. It is part of a gene family with eight members in Arabidopsis. EXO gene expression is under control of BR, and EXO overexpression promotes shoot and root growth. In this study, the consequences of loss of EXO function are described.

Results

The exo loss of function mutant showed diminished leaf and root growth and reduced biomass production. Light and scanning electron microscopy analyses revealed that impaired leaf growth is due to reduced cell expansion. Epidermis, palisade, and spongy parenchyma cells were smaller in comparison to the wild-type. The exo mutant showed reduced brassinolide-induced cotyledon and hypocotyl growth. In contrast, exo roots were significantly more sensitive to the inhibitory effect of synthetic brassinolide. Apart from reduced growth, exo did not show severe morphological abnormalities. Gene expression analyses of leaf material identified genes that showed robust EXO-dependent expression. Growth-related genes such as WAK1, EXP5, and KCS1, and genes involved in primary and secondary metabolism showed weaker expression in exo than in wild-type plants. However, the vast majority of BR-regulated genes were normally expressed in exo. HA- and GFP-tagged EXO proteins were targeted to the apoplast.

Conclusion

The EXO gene is essential for cell expansion in leaves. Gene expression patterns and growth assays suggest that EXO mediates BR-induced leaf growth. However, EXO does not control BR-levels or BR-sensitivity in the shoot. EXO presumably is involved in a signalling process which coordinates BR-responses with environmental or developmental signals. The hypersensitivity of exo roots to BR suggests that EXO plays a diverse role in the control of BR responses in the root.