Figure 4.

Developmental regulation of gene expression in wild strawberry shoot apices. The expression of AP1 (A), LFY (B), SOC1 (C) and GA3ox (D) in the SD and EB ('Baron Solemacher') genotype of the wild strawberry. Triplicate shoot apex samples were collected from LD grown plants at one to four leaf stage. Ct values were normalized against a Ubiquitin [GenBank:DY672326] gene to get normalized ΔCt values. The expression differences between one leaf stage and later developmental stages were calculated from the formula 2^{ΔCt later developmental stage}/2^{ΔCt one leaf stage}. The expression values at one leaf stage were artificially set to 1 separately for both genotypes. Values are mean ± SD. Note that Ubiquitin was amplified ~1 cycle earlier in SD genotype, but was stable between different developmental stages. Therefore, expression values between genotypes cannot be directly compared, while the expression levels between the various developmental stages are comparable.