Thylakoid protein profiles. Immunoblotting of wild-type and npq4 protein preparations from plants grown under normal (control) light (PPFD = 100 μmol quanta m-2 s-1) and high light (HL, PPFD = 1600 μmol quanta m-2 s-1) with antibodies against LOX-C and AOS. (A) total leaf extracts loaded on basis of equal leaf area in three dilutions, from left to right 1X, 2X and 3X. (B) thylakoid membrane proteins loaded on basis of equal protein – determined by bicinchoninic acid assay (Pierce)-, from left to right 20, 40 and 60 microgram protein.
Frenkel et al. BMC Plant Biology 2009 9:12 doi:10.1186/1471-2229-9-12