Research article
An archived activation tagged population of Arabidopsis thaliana to facilitate forward genetics approaches
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* Corresponding author: Isobel AP Parkin Isobel.Parkin@agr.gc.ca
1 Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, S7N 0X2, Canada
2 Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
3 Department of Biology, Biosciences Complex, Queens University, Kingston, Ontario, K7L 3N6, Canada
BMC Plant Biology 2009, 9:101 doi:10.1186/1471-2229-9-101
Published: 31 July 2009Additional files
Additional file 1:
The frequency of T-DNA integration is correlated with carpel tissue gene expression level but not recombination rate along the five A. thaliana chromosomes. These graphs demonstrate the relationship among the observed frequency of gene expression, T-DNA integration and genetic recombination observed along each of the five pseudochromosome molecules.
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Additional file 2:
Statistical analyses studying the impact of gene characteristics on T-DNA integration. Statistical analyses of the parameters gene expression, gene length, and proximity to the centromere for the two gene classes, those with and without an integration event. Additionally, statistical output are presented that demonstrates the impact of the presence of intron sequence, pseudogene classification and gene redundancy on T-DNA integration.
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Additional file 3:
Alignment of selected FST sequences to the A. thaliana genome. Blast output showing examples of: A) a simple T-DNA integration event into the A. thaliana genome; B) complex integration events described and numbered according to Figure 3.
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