Research article
A blackberry (Rubus L.) expressed sequence tag library for the development of simple sequence repeat markers
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* Corresponding author: Kim S Lewers kim.lewers@ars.usda.gov
1 USDA-ARS, Beltsville Agricultural Research Center, Genetic Improvement of Fruits and Vegetables Lab, Bldg. 010A, BARC-West, 10300 Baltimore Ave., Beltsville, MD 20705-2350, USA
2 Clemson University Genomics Institute, 51 New Cherry St., 304 Biosystems Research Complex, Clemson University, Clemson, SC 29634, USA
3 National Institutes of Health/National Institute of Environmental Health Sciences, Laboratory of Signal Transduction, Peptide Hormone Action Group, 111 TW Alexander Drive, PO Box 12233, MD F3-04 Research Triangle Park, NC 27709-2233, USA
4 Center for Integrated Biotechnology, Dept of Horticulture and Landscape Architecture, Washington State University, 45 Johnson Hall, Pullman, WA 99164-6414, USA
5 Monsanto Research Centre, Biotech Product Support, 44/2A Bellary Road, NH-7, Hebbal, Bangalore 560 092, India
BMC Plant Biology 2008, 8:69 doi:10.1186/1471-2229-8-69
Published: 20 June 2008Additional files
Additional file 1:
Blackberry (Rubus L.) EST contigs containing more than five sequences. Description and accession number of the Entrez protein database sequence that the blackberry sequence most closely matched (match sequence), and functional category of the match sequence.
Format: XLS Size: 22KB Download file
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Additional file 2:
Test of SSR primer pairs derived from blackberry (Rubus L.) SSR containing EST sequences. Included are contigs to which the sequences were assembled, sequence similarity matches, repeat motif within the sequence, number of repeats of the motif, placement in an open reading frame (ORF, "Yes" if the SSR was present in the ORF, and "No" if the SSR was not present in the ORF), amplification success, and type of polymorphism detected ("No" for no polymorphism, "Size" for a size polymorphism between the two genotypes tested, or "+/-" for a primer pair that amplified at least one product from one of the two genotypes but not the other).
Format: XLS Size: 23KB Download file
This file can be viewed with: Microsoft Excel Viewer