Additional file 5.


Changes in the microtubule array beneath the point of contact. Movie composed of 115 images of the microtubule array underneath the point of contact with a tungsten needle in A. thaliana expressing TUA6-GFP [70]. Each image in the movie is a projection of five optical sections through the cortical cytoplasm underlying the outer epidermal cell wall. The position of the needle is indicated by the V-shaped shadow (top centre). The movie commences just before touching the cell surface with the needle and ends 1 hour 17 minutes later. The time between most images is about 30–40 seconds. A bright diffuse cloud of GFP-tubulin begins to accumulate at the point of contact about 4 min after touching the cell with the needle. Linear arrays of diffuse fluorescence appear to sweep below the cortical microtubule array. The movie plays at about 200 times real-time.

Format: MOV Size: 12.8MB Download file

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Hardham et al. BMC Plant Biology 2008 8:63   doi:10.1186/1471-2229-8-63