Additional file 4.

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Dynamics of a bright cloud of actin at the point of contact. Movie composed of 73 images of the actin array underneath the point of contact with a tungsten microneedle in A. thaliana expressing hTalin-GFP. Each image in the movie is a projection of five optical sections through the cortical cytoplasm underlying the outer epidermal cell wall. The position of the needle is indicated by the V-shaped shadow. The movie commences just before touching the cell surface with the needle and ends 28 minutes later. The time between most images is about 20 seconds. Accumulation of actin below the point of contact forms a bright patch of fluorescence that appears to swirl around the needle tip. The movie plays at about 150 times real-time.

Format: MOV Size: 3.3MB Download file

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Hardham et al. BMC Plant Biology 2008 8:63   doi:10.1186/1471-2229-8-63