Additional file 2.

Formation, dispersal and re-formation of an actin patch at the contact site. Actin microfilaments visualized in the cortical cytoplasm underlying the outer epidermal cell wall in a cotyledon of A. thaliana expressing hTalin-GFP. The surface of the epidermal cell was touched with a glass microneedle at the site indicated by the asterisk in A. Times in minutes and seconds show elapsed time after the image in A. Images A-C show formation of a patch of actin microfilaments about 6 minutes after touching the cell surface. Needle contact was made about 10 minutes after the image in A was taken. Images D-F show dispersal of the actin patch after the needle lifted off the cotyledon. Images G-I show reformation of the actin patch when the needle was again brought into contact with the cell at the same location. The image in G was taken 1 minute after re-positioning the needle to touch the surface again. Images are projections of 7 (B, G-I), 8 (C, E), 9 (D), 12 (F) or 13 (A) optical sections. Bar = 10 μm.

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Hardham et al. BMC Plant Biology 2008 8:63   doi:10.1186/1471-2229-8-63