Indirect immunofluorescence detection of xyloglucan and pectic HG epitopes in TS tobacco stem internode showing higher magnification micrographs of region of pith parenchyma. a. LM15 binding to cell walls after pectate lyase pretreatment is most strong at points of cell adhesion at corners of an intercellular space (arrows). b. Same section as a shown combined with Calcofluor White fluorescence. c. Calcofluor White fluorescence of an intact cross wall in pith parenchyma – equivalent cell shown as an asterisk in a and b. d. Same cell as c showing LM15 binding. e. Combined image of c and d. Single-headed arrows in c, d and e indicate points of weaker Calcofluor White/stronger LM15 fluorescence. Double-headed arrows in c, d and e indicate points of stronger Calcofluor White/weaker LM15 fluorescence. f. Equivalent section immunolabelled with JIM5 showing epitope particularly abundant around the entire lining of intercellular spaces. Arrows indicate points of cell adhesion at corners of an intercellular space. g. Equivalent section to c. h. Same section as g showing JIM5 labelling. i. Combined image of g and h. Arrows in g, h and i indicate cell wall regions with weaker Calcofluor fluorescence. Scale = 10 μm.
Marcus et al. BMC Plant Biology 2008 8:60 doi:10.1186/1471-2229-8-60