Microarray analysis of LM15 binding to an array of plant cell wall polysaccharides and CDTA- and NaOH-solubilised isolates of Arabidopsis cell walls. Pectic and hemicellulosic polysaccharides from a range of species were microarrayed on nitrocellulose sheets and the relative binding of LM15 determined using an enzyme-linked secondary antibody detection system. Binding intensities were quantified and the horizontal scale indicates relative binding. The result shown is representative of at least three separate glycan profiling assays.
Marcus et al. BMC Plant Biology 2008 8:60 doi:10.1186/1471-2229-8-60