ELISA of rat monoclonal antibody LM15 binding to the XXXG-BSA glycoprotein used as immunogen, tamarind xyloglucan and mannan. Absorbance values shown are duplicate means with the standard deviations being ≤ 0.05 absorbance units. The result shown is representative of at least three separate experiments. LM15 bound effectively to the immunogen and tamarind xyloglucan but did not bind to ivory nut mannan. Polysaccharides were coated on to plates by incubation at 50 μg/ml. The LM15 hybridoma supernatant displayed effective recognition of the glycoprotein and tamarind xyloglucan when diluted 500-fold.
Marcus et al. BMC Plant Biology 2008 8:60 doi:10.1186/1471-2229-8-60