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Open Access Research article

Characterization of phenylpropanoid pathway genes within European maize (Zea mays L.) inbreds

Jeppe Reitan Andersen1, Imad Zein2, Gerhard Wenzel2, Birte Darnhofer3, Joachim Eder3, Milena Ouzunova4 and Thomas Lübberstedt1*

Author Affiliations

1 Department of Genetics and Biotechnology, University of Aarhus, Research Center Flakkebjerg, 4200 Slagelse, Denmark

2 Department of Agronomy and Plant Breeding, Technical University of Munich, Am Hochanger 2, 85354 Freising-Weihenstephan; Germany

3 Bavarian State Research Center for Agriculture, Vöttinger Str. 38, 85354 Freising-Weihenstephan, Germany

4 KWS Saat AG, Grimsehlstr. 31, 37555 Einbeck, Germany

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BMC Plant Biology 2008, 8:2  doi:10.1186/1471-2229-8-2

Published: 3 January 2008

Abstract

Background

Forage quality of maize is influenced by both the content and structure of lignins in the cell wall. Biosynthesis of monolignols, constituting the complex structure of lignins, is catalyzed by enzymes in the phenylpropanoid pathway.

Results

In the present study we have amplified partial genomic fragments of six putative phenylpropanoid pathway genes in a panel of elite European inbred lines of maize (Zea mays L.) contrasting in forage quality traits. Six loci, encoding C4H, 4CL1, 4CL2, C3H, F5H, and CAD, displayed different levels of nucleotide diversity and linkage disequilibrium (LD) possibly reflecting different levels of selection. Associations with forage quality traits were identified for several individual polymorphisms within the 4CL1, C3H, and F5H genomic fragments when controlling for both overall population structure and relative kinship. A 1-bp indel in 4CL1 was associated with in vitro digestibility of organic matter (IVDOM), a non-synonymous SNP in C3H was associated with IVDOM, and an intron SNP in F5H was associated with neutral detergent fiber. However, the C3H and F5H associations did not remain significant when controlling for multiple testing.

Conclusion

While the number of lines included in this study limit the power of the association analysis, our results imply that genetic variation for forage quality traits can be mined in phenylpropanoid pathway genes of elite breeding lines of maize.