Table 2

Specification of transcripts showing similarity to enzymes involved in starch and lipid reserve mobilization.a

Reaction

Enzyme

GenBank accession number of EST showing similarity to the enzyme

Cluster

Maximal absolute expression under standard conditions

Relative expression under standard conditions


Starch degradation

1

Alpha-amylase (EC 3.2.1.1)

FG345587

k2.2

4240

148.2

1

Alpha-amylase (EC 3.2.1.1)

FG344655

k2.1

1820

43.1

2

Beta-amylase (EC 3.2.1.2)

FG345593

-

490

5.1

2

Beta-amylase (EC 3.2.1.2)

FG344086

-

205

0.3

3

Alpha-glucosidase (EC 3.2.1.20)

FG344078

-

5

1.0

Glycolysis

4

Phosphoglucomutase (EC 5.4.2.2), cytosolic

BQ586122

-

70

1.0

5

UDP-glucose pyrophosphorylase (EC 2.7.7.9)

FG343121

-

110

3.2

6

Phosphofructokinase (PFK) (EC 2.7.1.11)

FG343255

k1.1

40

0.1

7

Pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP) (EC 2.7.1.90)

FG345703

k2.2

180

2.7

8

Fructose-bisphosphate aldolase (EC 4.1.2.1), cytosolic

FG343104

-

510

2.7

8

Fructose-bisphosphate aldolase (EC 4.1.2.1), plastidic

FG345732

k2.2

180

10.4

9

Triosephosphate isomerase (TPI) (EC 5.3.1.1), cytosolic

FG344285

k2.2

400

7.9

10

Glyceraldehyde-3-P dehydrogenase (GAPDH) (EC 1.2.1.12), cytosolic

FG345028

k2.2

1980

2.3

11

Phosphoglycerate kinase (PGK) (EC2.7.2.3), cytosolic

FG343147

k2.2

575

3.8

12

Phosphoglycerate mutase (PGAM) (EC 5.4.2.1), cytosolic

BQ583729

-

35

2.4

13

Enolase (ENO) (EC 4.2.1.11), cytosolic

FG345118

k2.1

440

4.1

14

Pyruvate kinase (PK) (EC 2.7.1.40), cytosolic

FG344735

k2.1

210

2.1

Pyruvate decarboxylation and fermentation

15

Pyruvate dehydrogenase E1 alpha subunit (EC 1.2.4.1)

FG345415

k2.1

80

4.9

16

Alcohol dehydrogenase (EC 1.1.1.1)

FG345166

k2.2

30

3.0

16

Alcohol dehydrogenase (EC 1.1.1.1)

FG343474

-

460

0.5

Fatty acid beta-oxidation

17

Acyl-CoA synthetase (EC 6.2.1.3)

FG344059

-

165

3.5

18

Acyl-CoA oxidase ACX3 (EC 1.3.3.6)

FG345045

-

120

1.5

19a

Enoyl-CoA hydratase (EC 4.2.1.17)

FG345194

k2.1

40

5.6

19b

Multifunctional protein (EC 4.2.1.17, EC 1.1.1.35)

FG344653

-

625

1.3

20

Thiolase (EC 2.3.1.9)

FG344716

k2.1

295

10.4

Glyoxylate cycle

21

ATP citrate synthase (EC 2.3.3.8)

FG345121

-

80

6.6

22

Aconitate hydratase (EC 4.2.1.3)

FG345009

k2.2

480

4.6

23

Isocitrate lyase, glyoxysomal (EC 4.1.3.1)

FG345539

k2.1

1970

9.4

24

Malate synthase, glyoxysomal (EC 2.3.3.9)

FG344826

-

690

2.5

25

Malate dehydrogenase (EC 1.1.1.37)

FG345685

k2.2

575

2.6

Gluconeogenesis

26

Phosphoenolpyruvate carboxykinase (EC 4.1.1.49)

FG343814

k2.2

475

15.3

Protein catabolism

-

Cysteine proteinase (EC 3.4.22.-)

FG344171

k2.1

1880

25.5

-

Cysteine-type endopeptidase (EC 3.4.22.1)

FG345664

k2.1

1700

85.0

-

Cysteine proteinase (EC 3.4.22.-)

FG345425

k2.2

505

50.5


a Enzymes and the corresponding transcripts are arranged according the metabolic scheme shown in Figure 5. Relative expression indicates maximal fold of increment during germination.

Pestsova et al. BMC Plant Biology 2008 8:122   doi:10.1186/1471-2229-8-122

Open Data