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Open Access Research article

Natural variation in CBF gene sequence, gene expression and freezing tolerance in the Versailles core collection of Arabidopsis thaliana

Heather I McKhann1*, Carine Gery2, Aurélie Bérard1, Sylvie Lévêque1, Ellen Zuther3, Dirk K Hincha3, S De Mita4, Dominique Brunel1 and Evelyne Téoulé25

Author Affiliations

1 Etude de Polymorphisme des Génomes Végétaux (EPGV), INRA, CNG, 2, rue Gaston Crémieux, 91057 EVRY Cedex, France

2 Station de Génétique et Amélioration des Plantes (SGAP), INRA, Route de Saint Cyr, 78026 Versailles Cedex, France

3 Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Potsdam, Germany

4 INRA, UMR 1097 "Diversité et Adaptation des Plantes Cultivées", Domaine de Melgueil, 34130 Mauguio, France

5 UPMC, 4 place Jussieu, 75252 Paris Cedex 05, France

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BMC Plant Biology 2008, 8:105  doi:10.1186/1471-2229-8-105

Published: 15 October 2008

Abstract

Background

Plants from temperate regions are able to withstand freezing temperatures due to a process known as cold acclimation, which is a prior exposure to low, but non-freezing temperatures. During acclimation, a large number of genes are induced, bringing about biochemical changes in the plant, thought to be responsible for the subsequent increase in freezing tolerance. Key regulatory proteins in this process are the CBF1, 2 and 3 transcription factors which control the expression of a set of target genes referred to as the "CBF regulon".

Results

To assess the role of the CBF genes in cold acclimation and freezing tolerance of Arabidopsis thaliana, the CBF genes and their promoters were sequenced in the Versailles core collection, a set of 48 accessions that maximizes the naturally-occurring genetic diversity, as well as in the commonly used accessions Col-0 and WS. Extensive polymorphism was found in all three genes. Freezing tolerance was measured in all accessions to assess the variability in acclimated freezing tolerance. The effect of sequence polymorphism was investigated by evaluating the kinetics of CBF gene expression, as well as that of a subset of the target COR genes, in a set of eight accessions with contrasting freezing tolerance. Our data indicate that CBF genes as well as the selected COR genes are cold induced in all accessions, irrespective of their freezing tolerance. Although we observed different levels of expression in different accessions, CBF or COR gene expression was not closely correlated with freezing tolerance.

Conclusion

Our results indicate that the Versailles core collection contains significant natural variation with respect to freezing tolerance, polymorphism in the CBF genes and CBF and COR gene expression. Although there tends to be more CBF and COR gene expression in tolerant accessions, there are exceptions, reinforcing the idea that a complex network of genes is involved in freezing tolerance and that the CBF genes alone cannot explain all differences in phenotype. Our study also highlights the difficulty in assessing the function of single transcription factors that are members of closely related gene families.