Table 3

Real-time quantitative RT-PCR results of the eight randomly selected ESTs from the SSH libraries.

Days (post inoculation)

Fold Change (infected/control)


Contig101

Contig852

Contig750

L11_67_Sh_CT

Contig748

Contig732

Contig710

Contig935


94-leaf-1-day

2.2 ± 1.5

1.0

-2.7 ± 0.0

1.0

1.0

1.0

1.0

1.0

94-leaf-3-weeks

3.7 ± 1.7

4.2 ± 1.0

1.0

1.0

2.4 ± 2.3

1.0

1.0

-2.7 ± 1.3

94-leaf-8-weeks

13.6 ± 1.0

10.1 ± 1.0

17.0 ± 1.2

1.0

15.9 ± 1.4

2.1 ± 1.7

1.0

1.0

67-leaf-1-day

-2.4 ± 0.8

-

1.0

1.0

-2.3 ± 0.8

3.8 ± 1.4

1.0

1.0

67-leaf-3-weeks

1.0

2.2 ± 1.2

1.0

1.0

1.0

1.0

1.0

-12.6 ± 1.1

67-leaf-8-weeks

1.0

-

-2.5 ± 1.5

1.0

1.0

1.0

1.0

1.0

94-stem-1-day

1.0

4.2 ± 6.2

1.0

1.0

1.0

-

1.0

6.6 ± 1.0

94-stem-3-weeks

30.3 ± 2.7

25.6 ± 1.2

23.5 ± 2.3

2.0 ± 0.7

5.2 ± 1.7

-118.6 ± 2.9

1.0

-56.5 ± 1.2

94-stem-8-weeks

464.7 ± 1.0

192.7 ± 1.1

94.0 ± 1.2

1.0

262.3 ± 1.2

1.0

1.0

1.0

67-stem-1-day

-2.4 ± 0.5

-2.7 ± 0.3

1.0

-4.5 ± 2.2

-2.0 ± 1.8

-6.6 ± 1.2

1.0

1.0

67-stem-3-weeks

2.5 ± 1.6

3.7 ± 1.3

34.2 ± 1.0

1.0

11.2 ± 1.8

-3.2 ± 3.5

1.0

1.0

67-stem-8-weeks

-4.4 ± 1.7

1.0

11.9 ± 3.3

1.0

3.0 ± 1.6

-8.6 ± 2.2

1.0

-2.9 ± 1.34


RNA from two tissues (stem and leaf) at three stages of development (1 day, 3 weeks and 8 weeks post infection) from both resistant (67) and susceptible (94) genotypes were analyzed. Results presented here are the mean ± SD values of biological replicates. Fold differences were calculated for Ct values of infected over control RNA samples. Values for less than two-fold change were entered as (1.0). For annotation and primer sequence details, please refer to Table-4.

Lin et al. BMC Plant Biology 2007 7:8   doi:10.1186/1471-2229-7-8

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