Table 1 |
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Primers for GATEWAY directional cloning |
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Primers |
Myosin XI-5 (At MYA1) |
Myosin XI-17 (At XI-K) |
|
|
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|
tail |
CACCCTTAGAATGCTGAAAATGGCTG |
CACCGCACGAGACACAGGAGC |
|
1/2 coil |
CACCGTTGTTGTTGAAGATACAG |
CACCGTATTGGTTGAGGATACTG |
|
no coil |
CACCACTCTTACCATCTCACCGAC |
CACCACCAGCAGAACTATGG |
|
1/2 tail |
CACCAGACGTCGGGGAATGCCTTC |
CACCCAGAGGCGAAGAACAACATCAGC |
|
dilute |
CACCGTGTTCGGGCAGATAT |
CACCGTATTCACACAAATATTCTCC |
|
reverse |
TCAATCTGACCTTTCCAACAAG |
TTACGATGTACTGCCTTCTTTACG |
|
|
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|
Myosin XI-6 (At MYA2) |
Myosin XI-15 (At XI-I) |
|
|
|
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|
tail |
CACCCTTAAGATGGCTGCTAGAG |
CACCCTTAAACAGGTTGCTAATG |
|
1/2 coil |
CACCGTGGTTGATCAGGAATT |
CACCGCGGTACTTGAAAAGC |
|
no coil |
CACCGTGAGGACAAATCTAGGAC |
CACCTCCCCAGAGAGAATAGGAC |
|
1/2 tail |
CACCGCTTCAACTTCTTTATTTGG |
CACCGCTTCAGCACTTCTATGC |
|
dilute |
CACCACTTTCTCATACATTAACG |
CACCCTTGTGACTCAGGTTTTCTC |
|
reverse |
CTAGTGCAAGAATACAAATGCTGG |
TCAAATGATCTGCTTTGAGGTTG |
|
|
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Myosin XI-8 (At XI-B) |
Myosin XI-16 (At XI-J) |
|
|
|
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tail |
CACCCTTAAGATGGCTGCTCG |
CACCTCAAAGCAGGCTGACAG |
|
reverse |
CTAGTGCAAGAATACGAATTCTGG |
TCAAAAGTAATCTTCGAAGCCC |
|
|
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All primers are listed 5'→3'. Forward primers contain the four bases CACC allowing directional cloning into pENTR/D TOPO vector (Invitrogen). |
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Reisen and Hanson BMC Plant Biology 2007 7:6 doi:10.1186/1471-2229-7-6 |
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