Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in vitro

doi:10.1186/1471-2229-6-7

BMC Plant Biology

Volume 6

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Research article

Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in vitro

Ravishankar Palanivelu¹ and Daphne Preuss²

¹Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA

²Howard Hughes Medical Institute, The University of Chicago, Chicago, IL 60637, USA

author email corresponding author email

BMC Plant Biology 2006, 6:7doi:10.1186/1471-2229-6-7


Published:	5 April 2006

Additional files

Additional File 1:

In vitro pollen tube guidance assay monitors ovule targeting by pollen tubes. Time-lapse imaging of GFP-tagged pollen tubes emerging from the cut portion of the pistil, travelling across the agarose medium before approaching a subset of excised ovules. In this instance, pollen tubes grew within 100 μm only near two of the six ovules; of these two, only one was successfully targeted.

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Additional File 2:

Pollen tube migration within an ovule. Time-lapse images showing a GFP-tagged tube entering and navigating within the ovule. The growth rate of the pollen tube decreases substantially before and soon after entry. Within the ovule, pollen tube ceases growth presumably in one of the synergids and tube discharge occurs, leaving a visible GFP spot.

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Additional File 3:

Pollen tube repulsion by a targeted ovule, example 1. Three pollen tubes approach an ovule, however only one of them is ultimately successful. The unsuccessful tubes stall near the micropyle, despite coming very close to it.

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Additional File 4:

Pollen tube repulsion by a targeted ovule, example 2. Three pollen tubes approach an ovule, however the pollen tube that arrived at the micropyle first was successful. The unsuccessful tubes approached the micropyle; however, one of them turned away from it, while the other one skips the micropyle and grows over the successful tube and stalls. The repulsion behaviors initiate well ahead of pollen tube discharge within ovule.

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