A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus
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* Corresponding author: Dario Grattapaglia dario@cenargen.embrapa.br
1 EMBRAPA Recursos Genéticos e Biotecnologia, CP 02372, 70770-970 DF Brasilia, Brazil
2 Department of Cell Biology, Universidade de Brasília UnB, DF, Brasília
3 EMBRAPA Arroz e Feijão, CP 179, Goiânia GO 74001-970, Brazil
4 CSIRO Forestry and Forest Products, POBox E4008, Kingston ACT 2604, Australia
5 Graduate Program in Genomic Sciences and Biotechnology, Universidade Catolica de Brasília, 70790-160 DF Brasilia, Brazil
BMC Plant Biology 2006, 6:20 doi:10.1186/1471-2229-6-20
Published: 22 September 2006Additional files
Additional file 1:
Consolidated list of 300 EMBRA microsatellite markers. Summary of all the information for the 230 new microsatellite markers reported in this study (EMBRA71 through EMBRA395) plus 70 published earlier (EMBRA1 through EMBRA70) [10,26] including the Genbank accession number of the original sequences from which the microsatellite primer pairs were designed. Information includes: the microsatellite EMBRA number, repeat motif, forward and reverse primer sequences, recommended annealing temperature (°C), size of expected product in basepairs, and linkage group assignment. Map position is described for 161 plus 4 duplicated loci, (see text), totalling 165 loci that were mapped in the E. grandis G44 × E. urophylla U28 mapping population. (n.s. = microsatellite not segregating in the mapping pedigree; n.a. = not available raw sequence data for submisison to Genbank).
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Additional file 2:
Genetic characterization of anchor microsatellite markers. Summary information on the genetic diversity of 35 microsatellites selected as anchor loci as evaluated on a panel of 32 unrelated individual trees of Eucalyptus sp. (Hexp = expected heterozigosity; Hobs = observed heterozigosity).
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