Figure 3.

Virtual cDNA arrays and clone redundancy in different libraries. A FASTA file containing all HF, HR and EDIT assembly sequences was used as the target in BLASTN comparisons with sequences from each library as query. Each target cDNA (3820 total) was assigned a color based on homology to sequences in different libraries; sequences hybridizing to HF probes were assigned red, those hybridizing to HR probes green, and those with EDIT probes blue. The color intensity reflected the BLASTN score with higher values assigned to greater homology. Colors were mixed when sequences were present in more than one library: those present in both the HF and HR libraries were yellow, in both the HF and EDIT libraries pink, and in the HR and EDIT libraries teal. Assembly sequences with homologies in all three libraries are represented as white.