Figure 3.

EPR spectra of CAO expressed in E. coli. Membrane fractions from cells induced to over-express CAO cDNA were collected and spectra were determined as described in Methods with microwave power at 5 mW. (A), Upper trace, untreated membrane fraction; 2 scans; lower trace, sample in upper trace after addition of Chl a (5 μM); 1 scan; temperature, 125 K. (B), Membrane fraction after wash with 0.1 mM EDTA; 1 scan; temperature, 14 K. Vertical axis represents first derivative of EPR absorption intensity (in arbitrary units) with respect to magnetic field.