Table 1

Arabidopsis lines used in this study. All lines contain mutations that primarily affect the composition of chloroplast membranes involved in photosynthesis.

Line

Biochemical defect

Primary affect on membrane composition

Reference

fad5

16:0 to 16:1 desaturation on sn-2 of all chloroplast galactolipids

Elevated 16:0; no 16:3 in chloroplast membranes

[32]

fad6

16:1 to 16:2 and 18:1 to 18:2 desaturation on sn-1 and sn-2 of all chloroplast lipids

Elevated 16:1 and 18:1; no 16:3, diminished 18:2, 18:3

[33]

fad7 fad8

16:2 to 16:3 and 18:2 to 18:3 desaturation on sn-1 and sn-2 of all chloroplast lipids

Elevated 16:2 and 18:2; diminished 16:3 and 18:3

[35]

act1

Plastid glycerol-3-phosphate acyltransferase (GPAT) activity; first step in the prokaryotic pathway

Slightly elevated 18:1; diminished levels of 16-C fatty acids (prokaryotic pathway)

[34]

act1 fad6

Double mutant containing both the act1 and fad6 mutations

Slightly elevated levels of 18:1 (than act1 alone); no elevation of 16:1 (as in fad6); prokaryotic pathway blocked

This work

act1⁺⁺

Plastid GPAT overexpressor

Similar to wild type. Slightly elevated levels of 16:3, reflecting slightly enhanced flux through prokaryotic pathway

This work

Falcone et al. BMC Plant Biology 2004 4:17   doi:10.1186/1471-2229-4-17