Email updates

Keep up to date with the latest news and content from BMC Plant Biology and BioMed Central.

Open Access Research article

The Arabidopsis LRR-RLK, PXC1, is a regulator of secondary wall formation correlated with the TDIF-PXY/TDR-WOX4 signaling pathway

Jiehua Wang1, Melis Kucukoglu2, Linbin Zhang2, Peng Chen3, Daniel Decker4, Ove Nilsson2, Brian Jones5, Göran Sandberg4 and Bo Zheng6*

Author Affiliations

1 School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China

2 Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden

3 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

4 Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, SE-901 87, Sweden

5 Faculty of Agriculture and Environment, Department of Plant and Food Sciences, University of Sydney, Sydney, Australia

6 College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China

For all author emails, please log on.

BMC Plant Biology 2013, 13:94  doi:10.1186/1471-2229-13-94

Published: 1 July 2013

Abstract

Background

Although a number of leucine-rich repeat receptor-like kinase-encoding genes (LRR-RLKs) have been identified in plants, a functional role has been determined for only a few. Recent studies have demonstrated that an LRR-RLK, PXY/TDR, is important for the process of secondary vascular development. Other studies have indicated that PXY/TDR is unlikely to be the sole LRR-RLK involved in this complex process.

Results

In this study, in silico analyses led to the identification of three Arabidopsis LRR-RLK genes (PXY-correlated; PXC1, 2, 3) with transcript accumulation profiles that correlated strongly with several key regulators of vascular development, including PXY/TDR, HB-8, REV, and CLE41. Expression profiling using qPCR and promoter:reporter lines indicated that all three PXC genes are associated with the vasculature. One in particular, PXC1 (At2g36570), had a strong correlation with PXY/TDR. Shifting pxc1 mutants from long-days to short-days showed that loss of the gene led to a dramatic reduction in secondary wall formation in xylem fibers. Transcript analysis of mutants for a variety of secondary cell wall-associated genes, including PXY/TDR indicated that the pathways mediated by PXC1 connect with those mediated by the TDIF-PXY/TDR-WOX4 system.

Conclusions

The data indicate that the LRR-RLK, PXC1 is involved in secondary cell wall formation in xylem fibers. Whereas further study is needed to identify the ligands and mode of action of the PXC1 protein, it is clear from this work that similarly to the shoot apical meristem (SAM), secondary vascular development requires contributions from a number of LRR-RLKs.

Keywords:
LRR-RLK; Arabidopsis; Secondary Wall Formation; TDIF-PXY/TDR-WOX4 Signaling