Open Access Research article

The cell morphogenesis ANGUSTIFOLIA (AN) gene, a plant homolog of CtBP/BARS, is involved in abiotic and biotic stress response in higher plants

Emma W Gachomo12, Jose C Jimenez-Lopez3, Sarah R Smith1, Anthony B Cooksey1, Oteri M Oghoghomeh1, Nicholas Johnson1, Lamine Baba-Moussa4 and Simeon O Kotchoni12*

Author Affiliations

1 Department of Biology, Rutgers University, 315 Penn St, Camden, NJ 08102, USA

2 Center for Computational and Integrative Biology, 315 Penn St, Camden, NJ 08102, USA

3 Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, Granada E-18008, Spain

4 Department of Biochemistry, University of Abomey-Calavi, Cotonou, Benin

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BMC Plant Biology 2013, 13:79  doi:10.1186/1471-2229-13-79

Published: 14 May 2013

Additional files

Additional file 1: Figure S1:

ANGUSTIFOLIA is evolutionary conserved across plant species. Comparison of ANGUSTIFOLIA full length amino acid sequences from different plant species are represented. The asterisks indicate the conserved amino acids across species. phosphorylation motifs detected in AN from Arabidopsis are indicated. Amino acids identical in all ANGUSTIFOLIA sequences are shaded in gray. At: Arabidopsis thaliana, Os: Oryza sativa, Sb: Sorghum bicolor, Zm: Zea mays, Mt: Medicago truncatula, Gm: Glycine max, AN: Angustifolia.

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Additional file 2: Figure S2:

The effect of latrunculin B (LatB) on wild type and an-t1 seedlings is indistinguishable. (A) Light-grown seedling phenotype of wild type and an-t1 at different concentrations of LatB. (B) Root phenotype of wild type and an-t1 at different concentrations of LatB. Bars = 5 mm.

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Additional file 3: Figure S3:

an-t1 knockout mutant is more sensitive to taxol treatment than wild type. (A-B) Light-grown phenotypes of wild type (A) and an-t1 (B) at different concentrations of taxol. (C) Root phenotype of wild type and an-t1 at different concentrations of taxol. Bars = 5 mm. Asterisks indicate significant differences between the mutant and the wild type. *P <0.05, Student’s t test.

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Additional file 4: Figure S4:

ANAP Protein interaction network generated using the ANGUSTIFOLIA protein (AT1G01510). (A) The ANAP framework of the interaction based upon the node relationship of the source database and direct interaction detection method is depicted. (B) A more comprehensive interaction of ANGUSTIFOLIA with several other proteins using a depth search mode (for indirect interaction searches) is generated.

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Additional file 5: Table S1:

The depth search protein interaction network generated using the ANGUSTIFOLIA protein (AT1G01510) as query. The total number of interactions detected by ANAP from experimental data and inference-based approaches supporting the interactions are depicted. The databases supporting the interactions are shown.

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Additional file 6: Table S2:

Selected members of Gene ontology (GO)-enriched protein interaction with Angustifolia clustered by cellular component. These proteins were selected from the extended interaction network (144 proteins) depicted in Additional file 4: Figure S4, B.

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