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Open Access Research article

DNA replication arrest leads to enhanced homologous recombination and cell death in meristems of rice OsRecQl4 mutants

Yong-Ik Kwon145, Kiyomi Abe2, Masaki Endo1, Keishi Osakabe3, Namie Ohtsuki1, Ayako Nishizawa-Yokoi1, Akemi Tagiri1, Hiroaki Saika1 and Seiichi Toki145*

Author affiliations

1 Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

2 Functional Plant Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

3 Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura, Saitama, 338-8570, Japan

4 Graduate School of Nanobioscience, Yokohama City University, 22-2, Seto, Kanazawa, Yokohama, 236-0027, Japan

5 Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka, Yokohama, 244-0813, Japan

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Citation and License

BMC Plant Biology 2013, 13:62  doi:10.1186/1471-2229-13-62

Published: 12 April 2013

Abstract

Background

Mammalian BLM helicase is involved in DNA replication, DNA repair and homologous recombination (HR). These DNA transactions are associated tightly with cell division and are important for maintaining genome stability. However, unlike in mammals, cell division in higher plants is restricted mainly to the meristem, thus genome maintenance at the meristem is critical. The counterpart of BLM in Arabidopsis (AtRecQ4A) has been identified and its role in HR and in the response to DNA damage has been confirmed. However, the function of AtRecQ4A in the meristem during replication stress has not yet been well elucidated.

Results

We isolated the BLM counterpart gene OsRecQl4 from rice and analyzed its function using a reverse genetics approach. Osrecql4 mutant plants showed hypersensitivity to DNA damaging agents and enhanced frequency of HR compared to wild-type (WT) plants. We further analyzed the effect of aphidicolin—an inhibitor of S-phase progression via its inhibitory effect on DNA polymerases—on genome stability in the root meristem in osrecql4 mutant plants and corresponding WT plants. The following effects were observed upon aphidicolin treatment: a) comet assay showed induction of DNA double-strand breaks (DSBs) in mutant plants, b) TUNEL assay showed enhanced DNA breaks at the root meristem in mutant plants, c) a recombination reporter showed enhanced HR frequency in mutant calli, d) propidium iodide (PI) staining of root tips revealed an increased incidence of cell death in the meristem of mutant plants.

Conclusions

These results demonstrate that the aphidicolin-sensitive phenotype of osrecql4 mutants was in part due to induced DSBs and cell death, and that OsRecQl4 plays an important role as a caretaker, maintaining genome stability during DNA replication stress in the rice meristem.

Keywords:
Rice; RecQ helicase; OsRecQl4; DNA replication; DNA double-strand break; Homologous recombination; Meristem