Open Access Research article

Vinyl sulfone silica: application of an open preactivated support to the study of transnitrosylation of plant proteins by S-nitrosoglutathione

Juan C Begara-Morales1, F Javier López-Jaramillo2*, Beatriz Sánchez-Calvo1, Alfonso Carreras1, Mariano Ortega-Muñoz2, Francisco Santoyo-González2, Francisco J Corpas3 and Juan B Barroso1

Author Affiliations

1 Grupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC (EEZ), Departamento de Bioquímica y Biología Molecular, Universidad de Jaén, de Jaén, Spain

2 Instituto de Biotecnología, Universidad de Granada, Granada, Spain

3 Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Granada, Spain

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BMC Plant Biology 2013, 13:61  doi:10.1186/1471-2229-13-61

Published: 12 April 2013

Additional files

Additional file 1: Figure S1:

Synthesis of the S-nitrosoglutathione-vinyl sulfone silica. Figure S2. Profile of the affinity chromatography of the pea leaves crude extract monitored at three wavelengths to detect both protein (A280) and S-nitrosothiol (A340 and A550) signal and the types of interactions that are disrupted. Figure S3. Bidimensional electrophoresis of the peak fraction of pea leaves eluted. Figure S4. Bidimensional electrophoresis of the peak fraction of sunflower hypocotyls. Table S1. Summary of the positive identifications by mass spectrometry of the peak fraction of the pea leave sample eluted from a GSNO-vinyl sulfone resin that showed the highest protein and Snitrosylation signals. Table S2. Summary of the positive identifications by mass spectrometry of the peak fraction of the sunflower hypocotyls sample eluted from a GSNO-vinyl sulfone resin that showed the highest protein and S-nitrosylation signals.

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