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Open Access Research article

Over-expression of microRNA171 affects phase transitions and floral meristem determinancy in barley

Julien Curaba1, Mark Talbot1, Zhongyi Li1 and Chris Helliwell2*

Author Affiliations

1 CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia

2 Current address: School of Agriculture and Food Systems, University of Melbourne, Parkville, Victoria, 3010, Australia

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BMC Plant Biology 2013, 13:6  doi:10.1186/1471-2229-13-6

Published: 7 January 2013

Additional files

Additional file 1:

miR171 precursor and mature sequences. Information retrieved from miRBase (release-17) and Schreiber et al., 2011 [34]. (A) pri-miR171a secondary structure obtained using MFOLD ( http://mfold.rna.albany.edu webcite), red letters indicate mature miRNA sequence. (B) mature miR171 sequences found in Hordeum vulgare (hvu), Arabidopsis thaliana (Ath) and Oryza sativa (Osa).

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Additional file 2:

Potential targets of hvu-miR171a and b. psRNAtarget ( http://plantgrn.noble.org/psRNATarget webcite) was used to predict potential targets of hvu-miR171a and b among the full-length barley ESTs assembled by Matsumoto et al., 2011 [35]. The maximum score was set at 4. The column “Activity” refers to the type of inhibition predicted, Cleavage (CL) or Translation inhibition (TR).

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Additional file 3:

Evidence for miR171 cleavage of HvSCLs and HvSPL. (A) Evidence for cleavage of top four predicted miR171 targets in degradome data from developing barley grains [36] (B) RLM-5 RACE on HvSCL (U21_33945). (C) RLM-5 RACE on HvSPL (U21_18637). The miRNA binding site is underlined in black and the 5 end of the cleaved mRNA in red. The numbers in green refer to the ratio of 5-RACE clones showing a cleavage at the base indicated by the arrow. The agarose gel on the left shows the band corresponding to the amplified 3 cleavage products.

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Additional file 4:

Timing of the transition phases during shoot development. The diagram shows the approximate timing of the transitions from juvenile to adult phases and adult to reproductive phases. The first transition was determined by the moment when the first stem of the plant started to elongate (jointing) and the second transition (flowering) when the first spike reached anthesis. Under our SD condition, OE171 plants did not flower.

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Additional file 5:

Developmental arrest of OE171-3 (T0). (A) Tiller of the T0 plant OE171-3. Scanning electron microscopy of the SAM of a OE171-3 (B) and WT (C) plants. Ectopic inflorescence meristem (eIM). Scale bars represent 1 mm.

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Additional file 6:

Comparison of vegetative WT and OE171 plants. Representative WT and OE171 plants at 4 weeks old in LD conditions.

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Additional file 7:

Primer and Probe sequences used in this study.

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